miR156a在不同海棠花瓣色泽形成中的作用  

The role of miR156a in the coloring of different Malus petals

作　　者：张洁 程昊 田佶[1] 张杰[1] 姚允聪[1] ZHANG Jie;CHENG Hao;TIAN Ji;ZHANG Jie;YAO Yuncong(Beijing Key Laboratory of New Technology in Agricultural Application/National Demonstration Center for Experimental Plant Production Education/Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]农业应用新技术北京市重点实验室/植物生产国家级实验教学示范中心/北京农学院植物科学技术学院,北京102206

出　　处：《北京农学院学报》2020年第4期43-47,共5页Journal of Beijing University of Agriculture

基　　金：长城学者项目(5045243001);北京农学院学位与研究生教育改革与发展项目(2019YJS038)。

摘　　要：【目的】为了揭示miR156a对观赏海棠花瓣花色苷形成的调控作用。【方法】以观赏海棠‘王族’、‘印第安魔力’、‘当娜’3个品种不同时期花瓣为试验材料,确定pre-miR156a基因序列,并对miR156a的靶基因进行预测。通过分光光度法和qRT-PCR分析花瓣中花色苷含量和miR156a相对表达量的差异。【结果】3个品种不同时期海棠花瓣中miR156a的含量有明显差异,miR156a随着花瓣增长相对表达量逐渐增多,随着颜色变深相对表达量逐渐减少。【结论】在海棠花瓣中,miR156a负调控花色苷生物合成。【Objective】To reveal the effect of miR156 a on anthocyanin formation in Malus petals. 【Methods】The test materials are different periods of Malus petals: ‘Royal’, ‘Indian magic’, and ‘Dana’ of Malus. This is for identifying the differences between the miR156 a gene sequence of petals and predict the target genes. The differences in anthocyanin content and relative expression of miR156 a in petals were analyzed by spectrophotometry and qRT-PCR. 【Results】The content of miR156 a of three varieties of Malus petals in different periods was significantly different. The relative expression of miR156 a increased gradually with the growth of petals, and gradually decreased with the increase of color. 【Conclusion】In Malus petals, miR156 a had a negative regulatory effect on anthocyanin synthesis.

关 键 词：海棠 花色苷 miR156a 

分 类 号：S664.2[农业科学—果树学]