多花黑麦草抗旱代谢通路挖掘  

Mining metabolic pathways in drought tolerance of Lolium multiflorum

作　　者：张彩峡 吴洪新[3] 毕玉芬[1] ZHANG Caixia;WU Hongxin;BI Yufen(College of Animal Science and Technology,Yunnan Agricultural Universtiy,Kunming 650201,Yunnan,China;Tibet Vocational Technical College,Lhasa 850000,Tibet,China;Institute of Grassland Research of CAAS,Hohhot 010010,Inner Mongolia,China)

机构地区：[1]云南农业大学动物科技学院,云南昆明650201 [2]西藏职业技术学院,西藏拉萨850000 [3]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《草业科学》2020年第8期1528-1536,共9页Pratacultural Science

基　　金：云南省重点研发计划项目(2018BB001-03);西藏自治区高等学校科研创新团队建设项目(西藏职业技术学院2014-2017)。

摘　　要：为了明确多花黑麦草(Lolium multiflorum)抗旱代谢机制相关的重要通路,本研究采用气相色谱质谱联用技术,以我国南方冬季主要轮作品种“蓝天堂”为研究材料,检测其干旱胁迫的主要代谢产物。结果表明:干旱胁迫下,多花黑麦草代谢产物显著变化,通过Agilent ChemiStation软件、NIST2011数据库、SIMCA-P 12.0软件等多种软件和数据库相结合识别分析得到19个重要代谢物,其中衣康酸、牛磺酸、蔗糖、葡萄糖、赖氨酸、异亮氨酸、酪氨酸、天冬酰胺、肌醇、1-氨基环丙烷基-1-羧酸10种代谢物浓度显著下调(P<0.05),1-氢吲哚、磷酸、果糖、柠檬酸、苹果酸、高丝氨酸、苏氨酸、苯丙氨酸、肌酸9种代谢物浓度显著上调表达(P<0.05)。使用MetaboAnalyst 4.0软件进行通路富集,可以得到显著(P<0.05)变化的9条通路:抗坏血酸和醛糖酸代谢、甘氨酸丝氨酸苏氨酸代谢、丙氨酸天冬氨酸谷氨酸代谢、三羧酸循环、半乳糖代谢、氨酰tRNA生物合成、精氨酸脯氨酸代谢、赖氨酸生物合成、氰基氨基酸代谢。这9条对干旱响应最显著的通路与多花黑麦草抗旱性密切相关。研究明确了多花黑麦草在干旱胁迫下的主要代谢产物,挖掘出与多花黑麦草抗旱相关的重要代谢通路,对探知多花黑麦草的抗旱代谢机制具有重要意义,为进一步在干旱半干旱地区推广多花黑麦草提供了理论依据。To identify the important pathways related to the drought-resistant metabolic mechanism of ryegrass,the main metabolites of blue heaven were detected and studied by gas chromatography-mass spectrometry(GC-MS)under natural drought stress.Using partial least squares discrimination analysis and principal components analysis could completely distinguish between the control and drought stress group;furthermore,there were 19 metabolomic markers obtained by the splot analysis.Abundance of 10 biomarkers decreased,namely itaconic acid,taurine,sucrose,glucose,lysine,isoleucine,tyrosine,asparagine,myo-inositol,1-aminocyclopropane-1-carboxylic acid.Abundance of nine biomarkers increased,namely fructose,phenylalanine,homoserine,1-hydrogen indole,threonine,phosphate,creatinine,citric acid,malic acid.Enriched pathway analysis by MetaboAnalyst 4.0 obtained nine significant metabolic pathways(P<0.05),namely Ascorbate and aldarate metabolism;Glycine,serine and threonine metabolism;Alanine,aspartate and glutamate metabolism;Citrate cycle(TCA cycle);Galactose metabolism;Aminoacyl-tRNA biosynthesis;Arginine and proline metabolism;Lysine biosynthesis;Cyanoamino acid metabolism.The nine pathways were associated with drought resistance of ryegrass.The main metabolic pathways of Lolium multiflorum under drought stress were identified through the study,which is of great significance to explore the mechanism of drought resistance metabolism of Lolium multiflorum and would provide reference for further promotion of Lolium multiflorum.

关 键 词：多花黑麦草 抗旱 代谢组 代谢组标志物 代谢通路 

分 类 号：S543.6[农业科学—作物学]