茴香秸秆替代玉米秸秆对湖羊瘤胃液挥发性脂肪酸、微生物区系和肌肉脂肪酸组成的影响  

Effect of fennel straw replacing corn stover on rumen fermentation parameters and intramuscular fatty acid composition of Hu sheep

作　　者：朱宝珍 高强 李飞[1] 李发弟[1,3] ZHU Baozhen;GAO Qiang;LI Fei;LI Fadi(State Key Laboratory of Grassland Agro-ecosystems/Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs/College of Pastoral Agriculture Science and Teconology,Lanzhou University,Lanzhou 730020,Gansu,China;Jinchang Jujia Ecological Agriculture Co.,Ltd.,737100,Gansu,China;Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province,Minqin 733300,Gansu,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室/兰州大学农业农村部草牧业创新重点实验室/兰州大学草地农业科技学院,甘肃兰州730020 [2]金昌居佳生态农业有限公司,甘肃金昌737100 [3]甘肃省肉羊繁育生物技术工程实验室,甘肃民勤733300

出　　处：《草业科学》2020年第8期1588-1597,共10页Pratacultural Science

基　　金：公益性行业(农业)科研专项“北方农作物秸秆饲用化利用技术研究与示范(201503134)”;甘肃现代农业(草食畜)产业技术体系(GARS-09);中央高校基本科研业务费专项资金(lzujbky-2019-38)。

摘　　要：为分析茴香(Foeniculum vulgare)秸秆替代玉米(Zea mays)秸秆对湖羊瘤胃液挥发性脂肪酸、微生物区系和肌肉脂肪酸组成的影响,本研究选取48只健康无病的湖羊公羔作为试验对象,根据体重相近原则和饲喂日粮分为对照组、50%替代组和100%替代组,每组16只羊,试验共包括14 d过渡期、7 d预饲期和84 d的正试期。试验结束后屠宰采集瘤胃液用以瘤胃发酵参数和微生物的测定,采集背最长肌用以肌肉脂肪酸的测定。结果表明:1)茴香秸秆替代100%玉米秸秆时显著降低了瘤胃中异丁酸的含量(P<0.05),其他挥发性脂肪酸及乙酸丙酸比差异不显著(P>0.05),茴香秸秆替代50%玉米秸秆时异丁酸的含量最高;2)茴香秸秆替代100%玉米秸秆时显著降低了瘤胃总菌的数量(P<0.05),对其他微生物数量无显著影响(P>0.05),而茴香秸秆替代50%玉米秸秆时瘤胃总菌的数量最高;3)茴香秸秆替代50%玉米秸秆时会显著增加肌肉中iso C16:0、iso C18:0、C18:3 n-3和CLA的含量(P<0.05),而C16:0、C18:0、C18:1 t9\t10\t11、C18:2 n-6t、C20:1、C22:0和SFA(饱和脂肪酸)的含量与对照组之间差异不显著(P>0.05)。综上所述,茴香秸秆替代50%玉米秸秆时在对湖羊瘤胃发酵参数和肌肉脂肪酸组成的影响中效果最优。The experiment was conducted to investigate the effects of fennel straw replacing corn stover on the rumen fermentation parameters and composition of intramuscular fatty acids in Hu sheep.Forty-eight healthy and disease-free male Hu lambs were selected and divided into control(corn stover),fennel and corn stover,and fennel straw groups.Each group contained 16 sheep,and the experiment included a 14-day transition period,7-day pre-feeding period,and an 84-day trial period.After the end of the experiment,the rumen fluid was collected to evaluate the rumen fermentation parameters and microorganisms,and the longissimus dorsi muscle was collected to determine intramuscular fatty acid content.The results showed that:1)when feeding fennel straw to sheep,the ratio of isobutyric acid in rumen significantly decreased(P<0.05)and the ratio of propionic acid decreased(P>0.05)compared to that of the control group,while isobutyric acid content was highest in the 50%substitution group;2)when feeding fennel straw to sheep,the total bacteria content was significantly lower than that of the other groups(P<0.05),while other rumen microorganisms decreased in the each group(P>0.05);3)when feeding the Hu sheep fennel straw and corn stover,a significant increase in iso C16:0,iso C18:0,C18:3 n-3,and CLA intramuscular content(P<0.05)was observed,while the content of C16:0,C18:0,C18:1 t9\t10\t11,C18:2 n-6t,C20:1,C22:0,and SFA decreased(P>0.05).In summary,the effect of a 50%replacement of fennel straw for corn stover on the rumen fermentation parameters and muscle fatty acid composition of Hu sheep was deemed to be best.

关 键 词：茴香秸秆 玉米秸秆 湖羊 瘤胃发酵参数 脂肪酸 

分 类 号：S826.5[农业科学—畜牧学]