红色诺卡菌细胞壁骨架中化学成分的鉴别与单糖含量测定  

Identification of chemical components and monosaccharide assay in Nocardia rubra cell wall skeleton

作　　者：万众 于丹 王菲 李伟[2] 张海 WAN Zhong;YU Dan;WANG Fei;LI Wei;ZHANG Hai(Department of Urology,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China;School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Department of Pharmacy,Shanghai First Maternity and Infant Hospital,Tongji University,Shanghai 201204,China)

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,上海200080 [2]山东中医药大学药学院,山东济南250355 [3]同济大学附属第一妇婴保健院药剂科,上海201204

出　　处：《药学实践杂志》2020年第5期423-430,共8页Journal of Pharmaceutical Practice

基　　金：上海市卫健委科研面上项目(201740217)。

摘　　要：目的分析鉴别红色诺卡菌细胞壁骨架(Nr-CWS)中的化学成分,并对其中的单糖成分进行分析测定。方法首先,采用UHPLC-Q-TOF/MS方法对Nr-CWS提取物中的化学成分进行分离与分析,通过与Metlin数据库中代谢物成分信息比对,快速鉴别其中化学成分。然后,对Nr-CWS提取物中多糖水解后衍生化,采用UHPLC-MS/MS法对单糖衍生物进行定量分析,测定单糖的含量。结果共鉴别出Nr-CWS提取物中的64个化学成分,主要包括氨基酸、单糖、脂肪酸等成分。此外,建立了8种单糖柱前衍生化的UHPLC-MS/MS含量测定方法,含量测定结果表明,Nr-CWS中阿拉伯糖和半乳糖的含量最高,说明阿拉伯糖、半乳糖是组成Nr-CWS中多糖的主要组成成分。结论通过对Nr-CWS中的主要化学成分进行了分离与分析,对多糖水解后的单糖成分进行含量测定,为今后开展Nr-CWS活性成分的筛选及药理作用机制的研究奠定基础。Objective To analyze and identify the chemical components in the Nocardia rubra cell wall skeleton(Nr-CWS),and to determine the contents of monosaccharides accurately.Methods The extract of Nr-CWS was separated and analyzed by UHPLC-Q-TOF/MS method.The chemical components were quickly identified by matching the data with the information in the Metlin database.The monosaccharide contents in the Nr-CWS extract were determined by UHPLC-MS/MS method after derivatization.Results A total of 64 chemical components were identified in the extract of Nr-CWS,including amino acids,monosaccharides and so on.A assay method for 8 monosaccharides by UHPLC-MS/MS was successfully established.The content of arabinose in Nr-CWS was the highest,followed by galactose,which indicated that the main polysaccharide components in Nr-CWS may be composed of these monosaccharides.Conclusion In this study,we analyzed the main chemical components of Nr-CWS,which are amino acids,fatty acids and so on.The content of monosaccharide after polysaccharide hydrolysis was determined by UHPLC-MS/MS.This will lay a foundation for the screening of the active components of Nr-CWS and the study of its pharmacological mechanism.

关 键 词：红色诺卡菌细胞壁骨架 化学成分 鉴别 单糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]