口腔溃疡膜有效成分提取方法的考察及含量测定  

Investigation of the extraction method and content determination of the active components from oral ulcer film

作　　者：陈顺 崔莉莉 高守红 陆文铨 宋江丽 王志鹏 CHEN Shun;CUI Lili;GAO Shouhong;LU Wenquan;SONG Jiangli;WANG Zhipeng(Department of Pharmacy,Changzheng Hospital Affiliated to Naval Medical University,Shanghai 200003,China;School of Chemical and Biological Engineering,Yichun University,Yichun 336000,China)

机构地区：[1]海军军医大学附属长征医院药学部,上海200003 [2]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《药学实践杂志》2020年第5期466-468,480,共4页Journal of Pharmaceutical Practice

摘　　要：目的考察不同溶剂对口腔溃疡膜中有效成分地塞米松磷酸钠和克霉唑的提取率,优化有效成分含量测定方法。方法采用不同提取溶剂(甲醇、水、70%甲醇)对口腔溃疡膜中的有效成分进行提取,用HPLC法进行含量测定,得到最佳提取方法。结果纯水对口腔溃疡膜中地塞米松磷酸钠和克霉唑有最好的提取效果。地塞米松磷酸钠在0.482~16.328μg/ml范围内呈良好的线性关系(r=0.9999),平均回收率为(103.97±1.02)%;克霉唑在5.0145~200.5800μg/ml范围内呈良好的线性关系(r=0.9998),平均回收率为(104.23±0.63)%。结论本研究建立的纯水提取方法操作简单,提取效率高,含量测定方法操作简便,准确性、重复性好。Objective To investigate the extraction methods for active components from oral ulcer film and optimize the determination methods of active components dexamethasone sodium phosphate and metronazole.Methods Different extraction solvents(methanol,water and 70%methanol aqueous)were applied to extract the active components dexamethasone sodium phosphate and metronazole from oral ulcer film,which contents were quantified by a HPLC method.Results the extraction solvent water had the best efficacy and more simpler compared to the other two solvents.Clotriazole showed a good linear relationship within 5.0145-200.5800μg/ml(r=0.9998),and the average extraction recovery was(104.23±0.63)%,and for dexamethasone sodium phosphate,a good linear relationship was obtained in the range of 0.482-16.328μg/ml(r=0.9999),and the average extraction recovery was(103.97±1.02)%.Conclusion The water extraction method established in this study was simple and efficient,which showed features of simplicity,accuracy and repeatable.

关 键 词：口腔溃疡膜 地塞米松磷酸钠 克霉唑 含量测定 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]