丙泊酚静脉麻醉对老年SD大鼠淋巴细胞亚群及海马组织功能的影响  

Effects of propofol intravenous anesthesia on immune function and hippocampal tissue function in elderly SD rats

作　　者：张燕 刘勇[2] 戚扬颂 ZHANG Yan;LIU Yong;QI Yang-song(Department of Anesthesiology,Hainan Modern Women and Children Hospital,Haikou Hainan 570000,China;Department of Anesthesiology,the Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 830000,China)

机构地区：[1]海南现代妇女儿童医院麻醉科,海南海口570000 [2]新疆医科大学第四附属医院麻醉科,新疆乌鲁木齐83000

出　　处：《临床和实验医学杂志》2020年第18期1912-1915,共4页Journal of Clinical and Experimental Medicine

基　　金：新疆维吾尔自治区科学技术厅(编号:2016D01C52)。

摘　　要：目的探讨丙泊酚静脉麻醉对老年SD大鼠淋巴细胞亚群及海马组织功能影响。方法选择60只SPF级老年SD雄性大鼠,根据随机数字表法分为两组,每组各30只。对照组大鼠给予利多卡因椎管内阻滞麻醉,观察组大鼠给予丙泊酚全身静脉麻醉,采用流式细胞术检测两组大鼠术后30 min以及术后1 d、3 d淋巴细胞亚群变化、酶联免疫吸附法检测术后1 d、3 d血清炎性指标白介素-1β(IL-1β)与白介素-6(IL-6)水平、western blot法检测术后3 d、7 d海马组织胶质纤维酸性蛋白(GFAP)表达,并采用末端标记法(TUNEL)细胞凋亡检测试剂盒海马组织细胞凋亡,比较两组大鼠上述指标的差异。结果两组大鼠术后30 min以及1、3 d的CD3^+T淋巴细胞、CD4^+T淋巴细胞占比都显著低于术前30 min,观察组也显著低于对照组,差异均有统计学意义(P<0.05);两组大鼠术后30 min以及术后1 d、3 d的CD8^+T淋巴细胞占比对比差异无统计学意义(P>0.05)。观察组大鼠术后1 d、3 d的血清IL-1β与IL-6表达水平都显著高于对照组,差异具有统计学意义(P<0.05)。观察组大鼠术后3 d、7 d的海马组织GFAP相对表达水平、细胞凋亡指数都显著高于对照组,差异具有统计学意义(P<0.05)。结论相对于椎管内阻滞麻醉,在老年SD大鼠中应用丙泊酚静脉麻醉可抑制淋巴细胞亚群功能,导致GFAP蛋白的表达与炎症因子的释放,从而促进海马组织的凋亡。Objective To investigate the effect of propofol intravenous anesthesia on lymphocyte subsets and hippocampal tissue function in elderly Sprague-Dawley(SD)rats.Methods Sixty SPF-grade old male SD rats were divided into two groups according to the random number table method,with 30 rats in each group.The rats in the control group were given lidocaine intraspinal block anesthesia,and the rats in the observation group were given general intravenous anesthesia with propofol.The rats in the control group were given lidocaine intraspinal block anesthesia,and the rats in the observation group were given general intravenous anesthesia with propofol.Flow cytometry was used to detect the changes of lymphocyte subsets in the two groups of rats at 30 min after surgery and at 1 and 3 days after surgery,enzyme-linked immunosorbent assay was used to detect serum inflammatory indexes interleukin-1β(IL-1β)and interleukin-6(IL-6)levels at 1 and 3 days after operation,western blot method was used to detect the expression of glial fibrillary acidic protein(GFAP)in hippocampus at 3 and 7 days after operation,and TUNEL Cell Apoptosis Detection Kit is used for hippocampal cell apoptosis to compare the differences in the above indicators between the two groups of rats.Results The ratios of CD3^+ T lymphocytes and CD4^+ T lymphocytes in the two groups of rats at 30 min and 1,3 d after surgery were significantly lower than those at 30 min before surgery.The observation group was also significantly lower than the control group,and the differences were statistically significant(P<0.05);there was no significant difference in the ratio of CD8^+ T lymphocytes between the two groups of rats at 30 min and 1,3 days after surgery(P>0.05).The postoperative 1 d and 3 d of levels of serum IL-1β and IL-6 in the observation group were significantly higher than those in the control group(P<0.05).The postoperative 3 d and 7 d of relative expression level and apoptosis index of GFAP protein in hippocampus of the observation group were significantly high

关 键 词：老年SD大鼠 静脉麻醉 丙泊酚 淋巴细胞亚群 海马组织 

分 类 号：R614[医药卫生—麻醉学]