苦豆碱对缺氧/复氧损伤的人脐静脉内皮细胞保护机制研究  

Protective mechanism of aloperine on hypoxia/reoxygenation injured human umbilical vein endothelial cells

作　　者：马会军[1] 朱米娜[1] 杜燕 MA Hui-jun;ZHU Mi-na;DU Yan(Department of Vasculocardiology,Xi’an No.1 Hospital,Shaanxi 710002,China)

机构地区：[1]西安市第一医院心血管内科,西安710002

出　　处：《天然产物研究与开发》2020年第9期1592-1598,共7页Natural Product Research and Development

基　　金：西安市第一医院心血管内科科室基金(ZDXA2018016)。

摘　　要：苦豆碱(aloperine,ALO)是一种具有抗炎、抗肿瘤及抗感染功效的生物碱,但其对人脐静脉内皮细胞缺氧/复氧损伤的影响尚不明确。本研究利用人脐静脉内皮细胞建立了体外缺氧/复氧损伤的细胞模型,对细胞进行分组处理:对照组、缺氧/复氧损伤组、苦豆碱(20、50和100μmol/L)预处理组。对细胞活力、细胞内乳酸盐脱氢酶(LDH)、丙二醛(MDA)和超氧化物歧化酶(SOD)活性,细胞内白细胞介素-1β(IL-1β)和肿瘤坏死因子α(TNF-α),以及内质网应激相关蛋白GRP78,XBP-1和凋亡相关蛋白CHOP的表达进行了检测。我们发现与缺氧/复氧损伤组相比,苦豆碱预处理能显著提高细胞活力和SOD活性、降低LDH活性、MDA含量以及IL-1β和TNF-α的水平(P<0.05)。另外,苦豆碱预处理还显著下调缺氧/复氧损伤引起的GRP78、XBP-1和CHOP水平的上升(P<0.05)。本研究证实苦豆碱能够提高细胞抗脂质过氧化反应的能力、降低炎症因子水平、抑制内质网应激引起的细胞凋亡,改善缺氧/复氧损伤引起的内皮细胞损伤。Aloperine(ALO)is an alkaloid with anti-inflammatory,anti-tumor and anti-infection activities.However,its protective effects on hypoxia/reoxygenation(H/R)injured human umbilical vein endothelial cells(HUVECs)and involved mechanisms are unclear.In this study,hypoxia/reoxygenation injured HUVECs were constructed in vitro.The cells were divided into control group,hypoxia/reoxygenation group(H/R group)and different concentrations of aloperine(20,50 and 100μmol/L)pretreated groups.Cell viability,the activity of lactate dehydrogenase(LDH)and superoxide dismutase(SOD),the content of methane dicarboxylic aldehyde(MDA),the level of intreleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α),and the protein expression of GRP78,XBP-1 and CHOP of different groups were measured.The results showed that compared with hypoxia/reoxygenation group,aloperine pretreatment could increase HUVECs viability and SOD,decreased LDH activity,MDA content,as well as levels of TNF-αand IL-1β(P<0.05).Aloperine pretreatment also down-regulated hypoxia/reoxygenation induced increase of GRP78,XBP-1 and CHOP(P<0.05).In summary,aloperine could improve the resistant of HUVECs against lipid peroxidation,decrease the levels of inflammatory cytokines and inhibit ERS related cell apoptosis,thus protect the HUVECs from hypoxia/reoxygenation injury.

关 键 词：苦豆碱 人脐静脉内皮细胞 缺氧/复氧 炎症 内质网应激 

分 类 号：R54[医药卫生—心血管疾病] R285[医药卫生—内科学]