市售乌梢蛇及其常见混淆品的鳞片微形态鉴别研究  

Identification of Scale Micromorphology of Zaocys Dhumnades(Cantor)and Its Common Adulterants on the Market

作　　者：郭利霄 齐兰婷 苏畅 郑倩 赵建成[4] 侯芳洁[1] 郑玉光 GUO Lixiao;QI Lanting;SU Chang;ZHENG Qian;ZHAO Jancheng;HOU Fangjie;ZHENG Yuguang(School of Pharmacy,Hebei University of Chinese Medicine,Traditional Chinese Medicine Processing Technology Innovation Center of Hebei province,Shijiazhuang,050090,China;Shijiazhuang Food and Drug Inspection Center,Shijiazhuang 050023,China;Chinese Traditional Medicine Hospital of Shijiazhuang,Shijiazhuang 050051,China;College of Life Sciences,Hebei Normal University,Shijiazhuang 050011,China;Hebei Chemical and Pharmaceutical College,Shijiazhuang 050023,China)

机构地区：[1]河北中医学院药学院,河北省中药炮制技术创新中心,石家庄050200 [2]石家庄市食品药品检验中心,石家庄050023 [3]石家庄市中医院,石家庄050051 [4]河北师范大学生命科学学院,石家庄050011 [5]河北化工医药职业技术学院,石家庄050026

出　　处：《中国现代应用药学》2020年第14期1698-1703,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：孙宝惠全国名老中医药专家传承工作室(7002016008005);河北省现代农业产业技术体系创新团队建设项目(HBCT2018060205);2018年校级教育教学改革研究项目(18yb-30);河北省大学生创新创业项目(201914432073)。

摘　　要：目的通过对乌梢蛇的头部及体部鳞片的微形态鉴别,将乌梢蛇与其常见的混淆品进行区分,为乌梢蛇药材和饮片的鉴别提供依据。方法用VHX-6000数码成像显微镜对乌梢蛇及其混淆品的头部鳞片进行3D观察,采用景深合成技术与大图拼接技术相结合,拍摄高清特征图。用光学显微镜对体部鳞片的端窝、薄膜囊、乳头突以及纵直纹理进行观察,并通过成像系统拍摄其特征图。最后通过DNA条形码技术对以上结果进行验证,保证微形态鉴别的准确性和可靠性。结果市场上乌梢蛇品种混乱,主要有以下几种伪品:滑鼠蛇、灰鼠蛇、Cerberus rynchops(Colubridae)、王锦蛇、黑眉锦蛇等,与之前报道的伪品有所不同。结论通过微形态鉴别能够很好区分乌梢蛇及其混淆品药材和饮片,并且鉴别特征稳定不易发生变化。OBJECTIVE To provide the basis for the identification of medicinal materials and decoction pieces of Zaocys dhumnades(Cantor)by identifying the scales of the head and body of Zaocys dhumnades(Cantor),Zaocys dhumnades(Cantor)and its common adulterants were distinguished.METHODS The scales of the head of Zaocys dhumnades(Cantor)and its adulterants were observed in 3-D with VHX-6000 digital imaging microscope,and the high-definition feature map was taken by combining depth-of-field synthesis technology with large-scale image stitching technology.The end fossa,thin film sac,papillary process and vertical texture of body scales were observed by optical microscope,and the characteristic images were taken by imaging system.Finally,the above results were verified by DNA barcode technology to ensure the accuracy and reliability of micro-morphological identification.RESULTS The variety of Zaocys dhumnades(Cantor)on the market was chaotic,mainly including the following fake products:Ptyas mucosa(Linnaeus),Ptyas korros(Schlegel),Cerberus Rynchops(Colubridae),Elaphe Carinata(Cunther),Orthriophis taeniurus Cope and so on,which were different from the fake products previously reported.CONCLUSION Through micro-morphological identification,the medicinal materials and decoction pieces of Zaocys dhumnades(Cantor)and its adulterants can be well distinguished,and the identification characteristics are stable and not easy to change.

关 键 词：乌梢蛇 3D景深合成技术 微形态鉴别 DNA鉴别 

分 类 号：R282.74[医药卫生—中药学]