UHPLC-ESI-MS/MS同时测定心安宁胶囊中的5种药效成分  

Simultaneous Determination of 5 Components in Xin’anning Capsules by UHPLC-ESI-MS/MS

作　　者：张婷[1] 王文彤[1] 陈玲[2] ZHANG Ting;WANG Wentong;CHEN Ling(Tianjin Institute of Medical and Pharmaceutical Sciences,Tianjin 300020,China;Affiliated Hospital of Tianjin Academy of TCM,Tianjin 300120,China)

机构地区：[1]天津市医药科学研究所,天津300020 [2]天津市中医药研究院附属医院,天津300120

出　　处：《中国现代应用药学》2020年第14期1730-1733,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立UHPLC-ESI-MS/MS同时测定心安宁胶囊中葛根素、二苯乙烯苷、金丝桃苷、槲皮素、齐墩果酸的含量。方法采用InertSustain■C18色谱柱(3.0 mm×100 mm,3.0μm),乙腈-0.1%甲酸水溶液,梯度洗脱,流速0.4 mL·min^-1,进样量10μL;质谱采用电喷雾离子源(ESI源),负离子扫描模式,多重反应监测模式进行定量分析。结果心安宁胶囊中5种成分在线性范围内峰面积与浓度均呈良好的线性关系,仪器精密度RSD<2.0%,平均加样回收率为99.15%~99.89%,RSD为0.89%~2.21%。在所设定的色谱条件下,心安宁胶囊中葛根素、二苯乙烯苷、金丝桃苷、槲皮素、齐墩果酸5个成分于10 min内完全分离。结论建立的UHPLC-ESI-MS/MS方法快速简便、精密度好、灵敏度高,可用于心安宁胶囊中多种成分的含量测定和质量控制。OBJECTIVE To develop a method for simultaneous determination of puerarin,2,3,5,4’-tetrahydroxystibene-2-O-β-D-glucoside,hyperoside,quercetin and oleanolic acid in Xin’anning capsules by UHPLC-ESI-MS/MS.METHODS The analysis was performed with triple quadrupole mass spectrometer by electrospray ionization(ESI-)mode and on InertSustain■C18chromatographic column(3.0 mm×100 mm,3.0μm).The mobile phase was acetonitrile and 0.1%formic acid-water with gradient elution at a flow rate of 0.4 mL·min^-1,and the injection volume was 10μL.RESULTS For the UHPLC-ESI-MS/MS assay,the calibration curve was linear,the precision of RSD was less than 2.0%,the average recoveries ranged from 99.15%to99.89%,RSD ranged from 0.89%to 2.21%.And under the optimized chromatographic conditions,the complete separation of 5 target components were obtained within 10 min.CONCLUSION The established UHPLC-ESI-MS/MS can be served as a scientific and effective way for quality control of Xin’anning capsules.

关 键 词：液质联用 心安宁胶囊 葛根素 二苯乙烯苷 金丝桃苷 槲皮素 齐墩果酸 多重反应监测 

分 类 号：R917.101[医药卫生—药物分析学]