Lys-GO对PLCL形状记忆纤维的力学增强和成骨诱导作用  

Effects of Lysine-Grafted Graphene Oxide on the Mechanical and Osteogenesis Properties of Shape Memory Capable PLCL Fibers

作　　者：刘畅 易兵成 王先流 沈炎冰[1] 秦春萍 张彦中[1] LIU Chang;YI Bingcheng;WANG Xianliu;SHEN Yanbing;QIN Chunping;ZHANG Yanzhong(College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《功能高分子学报》2020年第5期483-491,共9页Journal of Functional Polymers

基　　金：国家自然科学基金(31771050,31570969);国家重点研发计划项目(2016YFC1100203);东华大学中央高校基本科研业务费专项(2232019A3-09)。

摘　　要：将氧化石墨烯(GO)与具形状记忆特性的乳酸-己内酯共聚物(PLCL)混合电纺制备了GO/PLCL复合纤维。然后将碱性氨基酸—赖氨酸(Lys)通过化学方法与GO共价嫁接得到Lys-GO后电纺引入到PLCL纤维中,形成Lys-GO/PLCL复合纤维,并对其力学性能、形状记忆性能、酸度中和能力以及成骨分化效果等进行了表征和检测。结果表明:将GO引入到PLCL纤维中后,与PLCL膜相比,GO/PLCL纤维膜的杨氏模量提高了28.4%,形状回复力提高了28.3%;而Lys-GO的引入不仅在维持GO/PLCL纤维膜基本性能的前提下显著减缓PLCL的降解导致的酸性问题,而且增强了纤维支架的细胞相容性和成骨分化功效。Graphene oxide(GO)nanosheets with varied mass fractions were introduced into the shape memory capable poly(lactide-co-caprolactone)(PLCL)copolymer to prepare GO/PLCL composite nanofibers via electrospinning.Based on the tensile and shape memory properties of the produced GO/PLCL nanofiber films,an optimal GO mass fraction(m(GO)∶m(PLCL)),0.5%was determined to maximize the reinforcing effect of the GO nanosheets to the PLCL fiber matrix.Thereafter,an alkaline amino acid lysine(Lys)was selected to functionalize the GO(0.5%)via 1-(3-dimethy laminopropyl)-3-ethylcarbodiimide/N-hydroxysuccinimide chemistry followed by electrospinning for the generation of multifunctional Lys-GO/PLCL nanofibers.A series of characterization including mechanical properties,shape memory performance,acidity neutralization capacity and osteogenic differentiation were subsequently carried out with the produced Lys-GO/PLCL nanofibers.The results showed that among the four groups of GO/PLCL nanofibers loaded with different GO mass fractions(i.e.,0,0.5%,1%and 2%),incorporation of 0.5%GO within the PLCL nanofibers gave rise to the most remarkable enhancement efficiency,which was evidenced by 28.4%increase in Young's modulus and 28.3%increase in shape recovery stress.With the introduction of merely 0.5%of Lys-GO into the PLCL nanofibers,it was found that the fiber morphology,tensile properties and shape recovery stress of the GO/PLCL could be largely preserved(>85%).Most importantly,it was demonstrated that the developed Lys-GO/PLCL nanofibers not only enabled neutralize the acidic degradation products of the PLCL(e.g.,the pH acidity was neutralized to 5.2 for the Lys-GO/PLCL nanofibers,compared to that of the GO/PLCL counterpart with a pH of 4.2),but also possessed good cytocompatibility and osteogenic differentiation capacity in the mouse bone mesenchymal stem cells(rBMSCs).This newly developed shape memory capable Lys-GO/PLCL fibers may find applications in the construction of multifunctional bone tissue engineering scaffolds.

关 键 词：电纺 形状记忆纤维 氧化石墨烯 赖氨酸 骨组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]