雪旺细胞外泌体影响胶质瘢痕形成修复小鼠脊髓损伤的实验研究  

Schwann cell-derived exosomes promote functional recovery of spinal cord injury in mice via affecting glial scar formation

作　　者：潘大宇 宁广智[1] 冯世庆[1] PAN Da-yu;NING Guang-zhi;FENG Shi-qing(Department of Orthopedics,General Hospital,Tianjin Medical University,Tianjin Neurological Institute,Key Laboratory of Post-Neuroinjury Neuro-Repair and Regeneration in Central Nervous System,Ministry of Education and Tianjin City,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院骨科,天津市神经科学院,中枢神经系统神经损伤后神经修复和再生教育部重点实验室,天津300052

出　　处：《天津医科大学学报》2020年第5期397-400,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金国际合作与交流项目(81620108018);国家自然科学基金资助项目(81930070)。

摘　　要：目的:探讨雪旺细胞外泌体(SCDEs)对小鼠脊髓损伤的修复作用。方法:从小鼠坐骨神经中提取原代雪旺细胞,并将雪旺细胞培养上清分次超速离心得到雪旺细胞外泌体。透射电镜鉴定外泌体,尾静脉注射外泌体,每周2次,每次3μL/只,BMS评分观察小鼠行为学功能恢复,免疫荧光染色观察外泌体神经保护作用和胶质瘢痕变化情况。结果:SCDEs的直径范围为40~100 nm;与PBS组相比,SCDEs组行为学BMS评分增加(P<0.05),神经学功能指标有所恢复(P<0.01),胶质瘢痕增加(P<0.01)。结论:SCDEs可以促进损伤后胶质瘢痕的增加,修复脊髓损伤。Objective:To investigate the repair effect of schwann cell-derived exosomes(SCDEs)on spinal cord injury in mice.Methods:Primary schwann cells were extracted from the sciatic nerve of mice,and the schwann cell culture supernatant was subjected to ultracentrifugation to obtain SCDEs.Transmission electron microscopy was used to identify exosomes,and exosomes were injected via tail vein 2 times a week,3μL each mouse.Basso mouse scale(BMS)was used to observe the behavioral function,nerve function recovery and changes scar were observed by immunofluorescence staining.Results:SCDEs ranged in size from 40 to 100 nm.Compared with the PBS group,BMS was increased(P<0.05),neurological functional recovery was induced(P<0.001),glial scar formation was increased in the SCDEs group(P<0.001).Conclusion:SCDEs can promote the increase of glial scar and repairing spinal cord injuries.

关 键 词：脊髓损伤 雪旺细胞 外泌体 胶质瘢痕 

分 类 号：R68[医药卫生—骨科学]