胰岛素治疗对糖尿病大鼠阴茎组织中miR-126、VEGF、eNOS表达的影响  

Effect of insulin treatment on miR-126,VEGF and eNOS expression in penile tissues of diabetic rats

作　　者：胡睿 张威[1] 欧宁静 梁震 杨永姣 刘莉[1] 刘晓强 HU Rui;ZHANG Wei;OU Ning-jing;LIANG Zhen;YANG Yong-jiao;LIU Li;LIU Xiao-qiang(Department of Urology,General Hospital,Tianjin Medical University,Tianjin 300052,China;Department of Urology,The Second Hospital,Tianjin Medical University,Tianjin Institute of Urology,Tianjin 300211,China)

机构地区：[1]天津医科大学总医院泌尿外科,天津300052 [2]天津医科大学第二医院泌尿外科,天津市泌尿外科研究所,天津300211

出　　处：《天津医科大学学报》2020年第5期422-428,共7页Journal of Tianjin Medical University

基　　金：赵以成医学科学基金资助项目(ZYYFY2018031)。

摘　　要：目的:研究胰岛素治疗对糖尿病大鼠阴茎组织中miR-126、VEGF、eNOS表达的影响。方法:8周龄雄性Sprague-Dawley(SD)大鼠30只,采用随机数字表将其分为正常对照组(NC组)、糖尿病组(DM组)和胰岛素治疗组(Insulin组)。腹腔内注射链脲佐菌素(STZ)建立糖尿病(DM)大鼠模型,Insulin组于成模后开始注射胰岛素,连续8周。治疗结束后,检测各组大鼠的体重、血糖、阴茎海绵体内压(ICP)、平均颈动脉内压(MAP)及ICP与MAP比值(ICP/MAP),阴茎海绵体中内皮型一氧化氮合酶(eNOS)、血管内皮生长因子(VEGF)及miR-126的表达量。结果:胰岛素治疗后,Insulin组的体重较DM组明显增加(t=-10.481,P<0.001),血糖较DM组显著降低(t=4.356,P<0.001);Insulin组的ICP/MAP较DM组明显增加(t=-14.349,P<0.001);Western印迹结果显示,Insulin组阴茎海绵体中eNOS及VEGF蛋白表达量均较DM组显著增加(t=-6.786,-21.177,均P<0.001)。RT-PCR结果显示,Insulin组阴茎海绵体中eNOS、VEGF和miR-126的表达量均较DM组显著增加(t=-6.994、-8.238、-10.665,均P<0.001)。但上述指标均未达到NC组水平。结论:胰岛素治疗可增加阴茎组织中miR-126、VEGF及eNOS的表达水平。Objective:To investigate the effect of insulin treatment on miR-126,VEGF and eNOS expression in penile tissues of diabetic rats.Methods:30 male Sprague-Dawley(SD)rats aged 8 weeks were randomly divided into three groups by random number table:normal control group(NC group),diabetes mellitus group(DM group)and insulin treatment group(Insulin group).Intraperitoneal injection of streptozotocin(STZ)to establish a rat model of diabetes mellitus(DM).Insulin group were started to inject insulin for 8 weeks after the DM model established.When the treatment was finished,the body weight,blood glucose,intracorporeal pressure(ICP),mean arterial pressure(MAP)and the ratio of ICP and MAP(ICP/MAP)of rats in different groups were measured.The expressions of eNOS,VEGF and miR-126 in the penile tissues were detected.Results:After 8 weeks of insulin treatment,the body weight of Insulin group significantly increased compared with DM group(t=-10.481,P<0.001),while the blood glucose significantly decreased compared with DM group(t=4.356,P<0.001).The ICP/MAP of Insulin group significantly increased compared with DM group(t=-14.349,P<0.001).The expressions of eNOS and VEGF in Insulin group by Western blot were significantly increased compared with DM group(t=-6.786,-21.177,both P<0.001).The expressions of eNOS,VEGF and miR-126 in Insulin group by RT-PCR were significantly increased compared with DM group(t=-6.994,-8.238,-10.665,all P<0.001).However,the above results were not equal to the level of NC group.Conclusion:Insulin treatment can increase the expressions of miR-126,VEGF and eNOS in penile tissues.

关 键 词：糖尿病性勃起功能障碍 胰岛素 MIR-126 VEGF ENOS 

分 类 号：R698R587.2[医药卫生—泌尿科学]