白菜型油菜粒色主效基因BrTT1的调控机制分析  

Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L.

作　　者：王艳花 荐红举 邱晓 李加纳[1] WANG Yan-Hua;JIAN Hong-Jiu;QIU Xiao;and LI Jia-Na(College of Agronomy and Biotechnology,Southwest University/Chongqing Engineering Research Center for Rapeseed,Chongqing 400715,China;University of Saskatchewan,Saskatoon S7N5A8,Canada)

机构地区：[1]西南大学农学与生物科技学院/油菜工程研究中心,重庆400715 [2]萨斯喀彻温大学,加拿大萨斯卡通S7N5A8

出　　处：《作物学报》2020年第11期1678-1689,共12页Acta Agronomica Sinica

基　　金：中国博士后科学基金面上项目(2019M653319);重庆市自然科学基金博士后科学基金项目(cstc2019jcyj-bsh0102);高等学校学科创新引智基地项目(“111”项目)(B12006)资助。

摘　　要：白菜型油菜(Brassica rapa L.,2n=20,AA)为十字花科芸薹属作物,属于栽培油菜基本种。我国是白菜和白菜型油菜的起源中心,与甘蓝型油菜相比,其起源和栽培历史悠久,遗传资源丰富,具有天然而稳定的黄籽资源。大黄油菜是白菜型油菜中一种天然的黄籽资源,其粒色鲜黄,黄籽性状能够稳定遗传,且具有大粒、含油量高、自交亲和性好等优点,是研究油菜粒色性状的良好材料。本研究对白菜型油菜粒色主效基因BrTT1进行了进一步的功能验证,并对BrTT1的粒色调控机制进行了初步解析。序列比较结果表明,BrTT1在不同遗传背景白菜型油菜黄籽、红褐籽、黑籽品种中存在固定的差异位点,同一粒色材料所得BrTT1序列一致,可用于预测粒色表型;酵母双杂交分析表明,BrTT1可以与另外2个转录因子R2R3-MYB(BrTT2)和WD40(BrTTG1)以及一个催化酶(BrTT3)相互作用。qRT-PCR结果表明,超量和干扰BrTT1的表达,导致类黄酮合成路径结构基因及其他关键调节基因BrTT2、BrTTG1的异常表达或不表达,从而阻碍了原花色素的正常积累。研究结果进一步明确了BrTT1在白菜型油菜粒色形成中的调节活性。Brassica rapa(B.rapa L.,2n=20,AA)is a specie of Brassica genus,belonging to the basic species of cultivated rapeseed.China is the original center of Chinese cabbage and Brassica campestris.Compared with Brassica napus,it has a long history of origin and cultivation and rich genetic resources,which has natural and stable yellow seed resources.Dahuang has the natural yellow seed resource in B.rapa.Its seed coat color is bright yellow,the yellow seed trait can be stably inherited,and Dahuang has the advantages of large grain,high oil content and good self-adhesiveness.Sequence comparison showed that nucleotide polymorphisms were solely found in BrTT1 sequences from different seed color lines(yellow,red or brown,and black),which could be used to predict seed color phenotype.Yeast two-hybrid analysis indicated BrTT1 could interact with two other transcriptional factors R2R3-MYB(BrTT2)and WD40(BrTTG1),and one catalytic enzyme(BrTT3).Quantitative RT-PCR analysis of transgenic B.rapa lines with the gene down-regulated by RNA interference and up-regulated by overexpression revealed that two contrasting groups of genes were regulated by BrTT1 in the biosynthesis and deposition of flavonoids pigments in the seed of B.rapa.These results further define the regulatory activity of BrTT1 in seed coat color formation in Brassica species.

关 键 词：白菜型油菜 BrTT1 类黄酮代谢 酵母双杂 

分 类 号：S565.4[农业科学—作物学]