Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex  

作　　者：Takayuki Tohge Federico Scossa Regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie 

机构地区：[1]Max-Planck-lnstitut fiir Molekulare Pflanzenphysiologie,Am Muehlenberg 1,14476 Potsdam-Golm.Germany [2]Graduate School of Biological Science,Nara Institute of Science and Technology,Ikoma 630-0192 Japan [3]Council for Agricultural Research and Economics(CREA),Research Centre for Genomics and Bioinformatics,via Ardeatina 54600178 Rome,Italy [4]Universite de Toulouse,INP-ENSA Toulouse,Genomique et Biotechnologie des Fruits,Castanet-Tolosan 31326,France [5]Institute of Plant Systems Biology,4000 Plovdiv,Bulgaria [6]Max Planck Institute for Biology of Ageing,Joseph Stelzmann Strasse 9b,50931 Cologne,Germany [7]Institute of Botany and Molecular Genetics,BioSC,RWTH Aachen University,52056 Aachen,Germany [8]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Molecular Plant》2020年第7期1027-1046,共20页分子植物（英文版）

基　　金：T.T and A.R.F.gratefully acknowledge partial support by the Max Planck Society and NAIST(to T.T.)as well as the European Union Projects(TOMGEM,MultiBioPro,and PlantaSyst).Research activity of T.T.was additionally supported by the Alexander von Humboldt Foundation(7000228060 to T.T.);the JSPS KAKENHI Grant-in-Aid for Scientific Research B(19H03249 to T.T.);C(19K06723 to M.W.).

摘　　要：While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across species.In this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of tomato.For this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic genes.The combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid pathway.The combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit ripening.Our results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways.

关 键 词：Solanumly copersicum secondary metabolism natural diversity wild accessions pathway elucidation gene discovery 

分 类 号：Q94[生物学—植物学]