The miR319-Targeted GhTCP4 Promotes the Transition from Cell Elongation to Wall Thickening in Cotton Fiber  

作　　者：Jun-Feng Cao Bo Zhao Chao-Chen Huang Zhi-Wen Chen Ting Zhao Hong-Ru Liu Guan-Jing Hu Xiao-Xia Shangguan Chun-Min Shan- Ling-Jian Wang Tian-Zhen Zhang Jonathan F.Wendel Xue-Ying Guan Xiao-Ya Chen 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [2]Plant Stress Biology Center,Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [3]University of Chinese Academy of Sciences,Shanghai 200032,China [4]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China [5]Agronomy Department,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,Zhejiang 310058,China [6]Department of Ecology,Evolution and Organismal Biology,Iowa State University,Ames,IA 50011.USA [7]Plant Science Research Center,Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China

出　　处：《Molecular Plant》2020年第7期1063-1077,共15页分子植物（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31690092,31788103,31571251);the National Key R&D Program of China(2016YFD0100500);the Ministry of Agriculture of China(2016ZX08010002,2016ZX08005003).

摘　　要：Plant cell growth involves a complex interplay among cell-wall expansion, biosynthesis, and, in specific tissues, secondary cell wall (SCW) deposition, yet the coordination of these processes remains elusive. Cotton fiber cells are developmentally synchronous, highly elongated, and contain nearly pure cellulose when mature. Here, we report that the transcription factor GhTCP4 plays an important role in balancing cotton fiber cell elongation and wall synthesis. During fiber development the expression of miR319 declines while GhTCP4 transcript levels increase, with high levels of the latter promoting SCW deposition. GhTCP4 interacts with a homeobox-containing factor, GhHOX3, and repressing its transcriptional activity. GhTCP4 and GhHOX3 function antagonistically to regulate cell elongation, thereby establishing temporal control of fiber cell transition to the SCW stage. We found that overexpression of GhTCP4A upregulated and accelerated activation of the SCW biosynthetic pathway in fiber cells, as revealed by transcriptome and promoter activity analyses, resulting in shorter fibers with varied lengths and thicker walls. In contrast, GhTCP4 downregulation led to slightly longer fibers and thinner cell walls. The GhHOX3–GhTCP4 complex may represent a general mechanism of cellular development in plants since both are conserved factors in many species, thus providing us a potential molecular tool for the design of fiber traits.

关 键 词：cell elongation secondary cell wall cotton fiber Gossypium hirsutum TCP microRNA 

分 类 号：Q94[生物学—植物学]