基于UPLC-LTQ Orbitrap MS的益气降脂颗粒化学组成分析  

Chemical Composition Analysis of Yiqi Jiangzhi Granules Based on UPLC-LTQ Orbitrap MS

作　　者：张榆 胡少伟[1] 杨佳颖 张金华 王路 蒋丽娟 姜艳艳[1] 刘斌[1] ZHANG Yu;HU Shao-wei;YANG Jia-ying;ZHANG Jin-hua;WANG Lu;JIANG Li-juan;JIANG Yan-yan;LIU Bin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《质谱学报》2020年第5期411-426,I0001,共17页Journal of Chinese Mass Spectrometry Society

基　　金：北京市教委共建科研基地建设项目(基于药物动力学药物体系的中药复方质量控制与评价模式应用研究)资助。

摘　　要：建立了中药复方复杂成分快速鉴定的高效分析方法,并应用该方法对复方中药制剂益气降脂颗粒中的化学成分进行分析。通过超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱(UPLC-LTQ Orbitrap MS)扫描提供的化合物精确相对分子质量、多级碎片离子信息,与对照品的相对保留时间和质谱数据进行比对,并结合相关参考文献,实现对化合物结构的准确鉴定。共鉴定了益气降脂颗粒中97个化学成分,主要包括黄酮类、生物碱类、酚酸类、蒽醌类、三萜类、倍半萜类及其他类成分,其中小麦黄素、小麦黄素-7-O-葡萄糖苷、8-乙酰基-7-羟基-4-甲基香豆素、3-乙酰基香豆素为首次从益气降脂颗粒组方药味中鉴定得到。对益气降脂颗粒中多种化学成分的系统分析可以为其药效物质基础及质量控制研究提供科学理论依据。In order to comprehensively understand the chemical composition of Yiqi Jiangzhi Granules,ultra-high performance liquid chromatography-linear ion trap/electro-static orbitrap combined high resolution mass spectrometry(UPLC-LTQ Orbitrap MS)was applied.By comparing the exacted molecular mass,retention time and mass spectrometry fragment ion information of each component to reference substance and related literature,a total of 97 chemical composition were identified,including flavonoids,alkaloids,phenolic acids,anthraquinones,triterpenoids,sesquiterpenoids and other components.Besides,tricin,tricin-7-O-glucoside,8-acetyl-7-hydroxy-4-methylcoumarin and 3-acetylcoumarin were identified from the ten medicinal ingredients in Yiqi Jiangzhi prescription for the first time.Meanwhile,this study can provide a reference for the research of Yiqi Jiangzhi Granules quality control and pharmacodynamics development.

关 键 词：益气降脂颗粒 超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱(UPLC-LTQ Orbitrap MS) 黄酮类 蒽醌类 生物碱类 

分 类 号：O657.63[理学—分析化学]