Pax6和Wnt7a在小鼠角膜中的表达分析  

Expression analysis of Pax6 and Wnt7a in mouse cornea

作　　者：高杰[1] 穆尧 向松 丁玲[1] 胡蓉[1] 李红[1] 苏敏[1] GAO Jie;MU Yao;XIANG Song;DING Ling;HU Rong;LI Hong;SU Min(Department of Histology and Embryology,Guizhou Medical University,Guiyang Guizhou 550004,China)

机构地区：[1]贵州医科大学组织学与胚胎学教研室,贵州贵阳550004

出　　处：《局解手术学杂志》2020年第9期689-692,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：贵州省科学技术项目(黔科合基础[2020]1Y408);贵州省科技厅学术新苗培养项项目(黔科合平台人才[2018]5779-21);贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]201);贵州省科学技术基金项目(黔科合J字[2012]2037号)。

摘　　要：目的观察Pax6和Wnt7a在小鼠角膜中的表达及分布情况,探讨Pax6和Wnt7a在角膜中的关系。方法对小鼠与人、家兔、恒河猿的Pax6和Wnt7a氨基酸序列同源性进行生物信息学分析;石蜡切片法观察小鼠眼球壁的形态结构;免疫组织化学技术和流式细胞术分别检测Pax6和Wnt7a在小鼠角膜中的分布和表达。结果小鼠和人、家兔、恒河猿的Pax6和Wnt7a蛋白氨基酸序列同源性都超过90%,其中小鼠和人的同源性最高。HE染色显示了正常小鼠的眼球壁结构。Pax6的免疫阳性表达在小鼠角膜全层的细胞质和细胞膜上,阳性表达率约为77.84%;Wnt7a的免疫阳性主要表达在小鼠角膜全层的细胞膜上,阳性表达率约为63.51%。结论Pax6和Wnt7a均在小鼠角膜中高表达,可能参与角膜的形成。Objective To observe the expression and distribution of Pax6 and Wnt7a in mouse cornea,and explore the relationship between Pax6 and Wnt7a in the cornea.Methods The bioinformatic analysis were conducted on the amino acid sequence homology of Pax6 and Wnt7a from mouse,human,rabbit and macaca mulatta.The paraffin section method was used to observe the morphological structure of the eyeball wall of the mouse.The immunohistochemistry and the flow cytometry were used to detect the distribution and expression of Pax6 and Wnt7a in the mouse cornea respectively.Results The amino acid sequence homology of Pax6 and Wnt7a proteins among mouse,human,rabbit and macaca mulatta were more than 90%,and the homology between mouse and human was the highest.HE staining results showed the normal structure of eyeball wall in mice.The immunopositive of Pax6 was expressed on the cytoplasm and cell membrane of the entire layer of the mouse cornea,and the positive expression rate was about 77.84%.The immunopositive of Wnt7a was mainly expressed on the cell membrane of the entire layer of the mouse cornea,and the positive expression rate was about 63.51%.Conclusion Both Pax6 and Wnt7a are highly expressed in mouse cornea,which may be involved in the corneal formation.

关 键 词：PAX6 Wnt7a 角膜 小鼠 石蜡切片法 免疫组织化学技术 流式细胞术 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]