Pseudomonas sp.TK35-L enhances tobacco root development and growth by inducing HRGPnt3 expression in plant lateral root formation  

作　　者：CAO Yuan-yuan NI Hai-ting LI Ting LAY Khien-duc LIU Dai-song U Kang-miao TANG Xin-yun WANG Xiao-bo QIU Li-juan 

机构地区：[1]School of Life Sciences,Anhui Agricultural University,Hefei 230036,P.R.China [2]Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention,Anhui Agricultural University,Hefei 230036,P.R.China [3]Shiyan Municipal Tobacco Company of Hubei Province,Shiyan 442000,P.R.China [4]School of Agronomy,Anhui Agricultural University,Hefei 230036,P.R.China [5]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Bejing 100081,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第10期2549-2560,共12页农业科学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(41401269);the Key Project of the University Natural Science Research Project of Anhui Province,China(KJ2019A0183);the Open Fund of Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention(FECPP201902);the Key Research Project of China National Tobacco Corporation Hubei Company(027Y2020-011).

摘　　要：Rhizosphere colonization is a key requirement for the application of plant growth-promoting rhizobacteria(PGPR)as a bioferilizer.Signaling molecules are often exchanged between PGPR and plants,and genes in plants may respond to the action of PGPR.Here,the luciferase luxAB gene was electrotransformed into Pseudomonas sp.strain TK35,a PGPR with an afinity for tobacco,and the labelled TK35(TK35-L)was used to monitor colonization dynamics in the tobacco rhizosphere and evaluate the effects of colonization on tobacco growth and root development.The transcript levels of the hydroxyproline rich glycoprotein HRGPnt3 gene,a lateral root induction indicator,in tobacco roots were examined by qPCR.The results showed that TK35-L could survive for long periods in the tobacco rhizosphere and colonize new spaces in the tobacco rhizosphere following tobacco root extension,exhibiting significant increases in root development,seedling growth and potassium accumulation in tobacco plants.The upregulation of HRGPnt3 transcription in the inoculated tobacco suggested that TK35-L can promote tobacco root development by upregulating the transcript levels of the HRGPnt3 gene,which promotes tobacco seedling growth.These findings lay a foundation for future studies on the molecular mechanism underlying the plant growth-promoting activities of PGPR.Futhermore,this work provided an ideal potential strain for biofertilizer production.

关 键 词：Pseudomonas sp.TK35-L rhizosphere colonization TOBACCO growth promotion root development HRGPnt3 

分 类 号：S572[农业科学—烟草工业]