Decreasing detection frequency of MITE(MCLas-A)in the population of‘Candidatus Liberibacter asiaticus'recently collected in southern China  

作　　者：CUI Xue-jin ZENG Chun-hua LIU Ke-hong TENG Cai-ling ZHOU Chang-yong WANG Xue-feng 

机构地区：[1]National Citrus Engineering Research Center,Citrus Research Institute,Chinese Academy of Agricultural Sciences/Southwest University,Chongqing 400712,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第10期2597-2601,共5页农业科学学报（英文版）

基　　金：Funded by the National Natural Science Foundation of China(31671992,31871925);the National Key R&D Program of China(2018YFD0201500);the Key R&D Program of Guangxi,China(Gui Ke AA18118046).

摘　　要：An active miniature inverted-repeat transposable element(MITE),MCL as-A,was previously identified from'Candidatus Liberibacter asiaticus'known to be associated with citrus Huanglongbing(HL B,yellow shoot disease).To explore the recent transposition status of MCLas-A,389'Ca.L.asiaticus'strains collected from nine regions in China were amplifed using a specific primer set and three representative'Ca.L.asiaticus'strains were analyzed by next-generation sequencing(NGS)approach.PCR and genomic analysis showed that the entire MCLas A was only present in 1.80%(7/389)and the jumping-out type of the MITE was predominant(8123%)in samples tested,suggesting high frequency transposition occurred in'Ca.L.asiaticus'strains recently collected from China.Biological roles of transposition of the active MITE remain to be determined.

关 键 词：HUANGLONGBING Candidatus Liberibacter asiaticus' miniature inverted-repeat transposable element MITE 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]