健康儿童末梢血与静脉血血常规和网织红细胞相关参数检测结果的差异探讨  

Discussion on the differences in the detection results of blood routine and reticulocyte related parameters in peripheral blood and venous blood of healthy children

作　　者：金芳[1] 宋文琪[1] 李启亮[1] JIN Fang;SONG Wenqi;LI Qiliang(Laboratory Center,Beijing Children′s Hospital,Capital Medical University/National Center for Children′s Health,Beijing 100045,China)

机构地区：[1]国家儿童医学中心/首都医科大学附属北京儿童医院检验中心,北京100045

出　　处：《国际检验医学杂志》2020年第18期2186-2189,共4页International Journal of Laboratory Medicine

基　　金：国家卫生和计划生育委员会医政医管局委托项目[国卫医医护便函(2017)374号]。

摘　　要：目的探讨健康儿童静脉血与末梢血血常规和网织红细胞相关参数检测结果的差异。方法同时采集130例健康儿童的静脉血(静脉采血组)与末梢血(末梢采血组),采用日本SYSMEX XNL-350全自动五分类血细胞分析仪分别检测静脉血与末梢血的血常规和网织红细胞相关参数。比较两组血常规和网织红细胞相关参数水平,对两组检测结果进行偏移评估。结果末梢采血组中性粒细胞百分比、中性粒细胞绝对值、红细胞计数、血红蛋白、红细胞比容、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度、网织红细胞百分比、网织红细胞绝对值、高荧光强度网织红细胞百分比、网织红细胞血红蛋白含量均高于静脉采血组,淋巴细胞百分比、淋巴细胞绝对值、嗜碱性粒细胞绝对值、嗜碱性粒细胞百分比、血小板计数、平均红细胞体积均低于静脉采血组,差异有统计学意义(P<0.05);两组白细胞计数、单核细胞百分比、单核细胞绝对值、嗜酸性粒细胞绝对值、嗜酸性粒细胞百分比、未成熟网织红细胞百分比、中荧光强度网织红细胞百分比、低荧光强度网织红细胞百分比比较,差异均无统计学意义(P>0.05)。结论健康儿童静脉血与末梢血部分血常规和网织红细胞相关参数的检测结果存在差异,提示临床应针对儿童血常规、网织红细胞相关参数检测的不同标本类型分别建立参考区间,以提高临床诊疗的准确性。Objective To discuss the differences in the detection results of blood routine and reticulocyte related parameters in peripheral blood and venous blood of healthy children.Methods Venous blood(venous blood collection group)and peripheral blood(peripheral blood collection group)of 130 healthy children were collected at the same time.The blood routine and reticulocyte related parameters in venous blood and peripheral blood were detected by Japan SYSMEX XNL-350 automatic five classification hematology analyzer.The blood routine and reticulocyte related parameters were compared between the two groups,and the migration of the detection results of the two groups was evaluated.Results The neutrophil percentage,absolute value of neutrophil,red blood cell count,hemoglobin,hematocrit,mean corpuscular hemoglobin content,mean corpuscular hemoglobin concentration,reticulocyte percentage,absolute value of reticulocyte,high fluorescence intensity reticulocyte percentage and reticulocyte hemoglobin content in peripheral blood collection group were higher than those in venous blood collection group,the lymphocyte percentage,absolute value of lymphocyte,absolute value of basophil,basophil percentage,platelet count and mean corpuscle volume in peripheral blood collection group were lower than those in venous blood collection group,the difference was statistically significant(P<0.05).There was no significant difference in white blood cell count,monocyte percentage,absolute value of monocyte,absolute value of eosinophil,eosinophil percentage,immature reticulocyte percentage,medium fluorescence intensity reticulocyte percentage and low fluorescence intensity reticulocyte percentage between the two groups(P>0.05).Conclusion There are differences in the detection results of some blood routine and reticulocyte related parameters between venous blood and peripheral blood of healthy children.It is suggested that reference intervals should be established for different specimen types of children′s blood routine and reticulocyte related par

关 键 词：静脉血 末梢血 儿童 血常规 网织红细胞 

分 类 号：R446.11[医药卫生—诊断学]