气动管道传输系统对凝血及血小板功能指标检测结果的影响  

The influence of pneumatic pipeline transmission system on the detection results of blood coagulation and platelet function indexes

作　　者：张丽敏[1,2] 欧珠 李虓 孙越红[1,2] 王丹 王平[1,2] 赵晖 康熙雄[1,2] 张国军 ZHANG Limin;OU Zhu;LI Xiao;SUN Yuehong;WANG Dan;WANG Ping;ZHAO Hui;KANG Xixiong;ZHANG Guojun(Department of Clinical Laboratory,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;Beijing Immunological Reagents Clinical Engineering Research Center, Beijing 100070,China;Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京天坛医院检验科,北京100070 [2]北京市免疫试剂临床工程技术研究中心,北京100070 [3]首都医科大学,北京100069

出　　处：《国际检验医学杂志》2020年第18期2194-2197,共4页International Journal of Laboratory Medicine

基　　金：北京天坛医院2016年度院青年科研基金项目(2016-YQN-09);首都医科大学科研培育基金项目(PYZ2017067)。

摘　　要：目的观察气动管道传输系统(PTS)对凝血及血小板功能指标检测结果的影响。方法选取80例患者为研究对象,所有研究对象均同时抽取两管枸橼酸钠抗凝的静脉血,其中1管由人工传输至实验室(人工传输组),另1管经PTS传输至实验室(PTS传输组)。采用凝固法检测活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、纤维蛋白原(FIB)及凝血酶时间(TT);采用乳胶免疫比浊法检测D-二聚体(D-D)和纤维蛋白降解产物(FDP);采用光学比浊法检测血小板功能指标,包括花生四烯酸(AA)、二磷酸腺苷(ADP)和胶原(COL)诱导的血小板聚集率(PAG),即PAG-AA、PAG-ADP和PAG-COL。结果PTS传输组PT、APTT均低于人工传输组(P<0.05);FIB、TT、D-D和FDP在两组间比较,差异均无统计学意义(P>0.05)。PTS传输组PAG-AA高于人工传输组(P<0.05),PAG-ADP、PAG-COL低于人工传输组(P<0.05)。阿司匹林治疗有效患者中,两组PAG-AA比较,差异无统计学意义(P>0.05);阿司匹林治疗无效患者中,人工传输组PAG-AA高于PTS传输组(P<0.05)。氯吡格雷治疗有效及治疗无效患者中,人工传输组PAG-ADP均高于PTS传输组(P<0.05)。结论PTS可用于凝血功能检测标本的传输,不适用于血小板功能检测标本的传输;PTS对血小板功能检测结果的影响可能与抗血小板药物的疗效有关。Objective To observe the influence of pneumatic pipeline transmission system(PTS)on the detection results of blood coagulation and platelet function indexes.Methods A total of 80 patients were selected as the research objects,and all the research objects were drawn two tubes of sodium citrate anticoagulated venous blood at the same time.One tube was manually transferred to the laboratory(manual transfer group),and the other tube was transferred to the laboratory by PTS(PTS transfer group).The coagulation method was used to detect activated partial thromboplastin time(APTT),prothrombin time(PT),fibrinogen(FIB)and thrombin time(TT);The latex immunoturbidimetric method was used to detect D-dimer(D-D)and fibrin degradation products(FDP).The optical turbidimetric method was used to detect platelet function indexes,which were arachidonic acid(AA),adenosine diphosphate(ADP)and collagen(COL)induced platelet aggregation rate(PAG),induding PAG-AA,PAG-ADP and PAG-COL.Results The PT and APTT of the PTS transfer group were lower than those of the manual transfer group(P<0.05).The comparison of FIB,TT,D-D and FDP between the two groups showed no significant difference(P>0.05).The PAG-AA of the PTS transfer group was higher than that of the manual transfer group(P<0.05),PAG-ADP and PAG-COL of the PTS transfer group were lower than those of the manual transfer group(P<0.05).In patients with effective aspirin treatment,there was no significant difference on PAG-AA between the two groups(P>0.05).In patients with ineffective aspirin treatment,PAG-AA of the manual transfer group was higher than that of the PTS transfer group(P<0.05).In the patients with clopidogrel effective or ineffective,PAG-ADP of the manual transfer group was higher than that of the PTS transfer group(P<0.05).Conclusion PTS could be used for the transfer of blood coagulation function detection samples,but not for platelet function detection samples.The impact of PTS on platelet function detection results might be related to the efficacy of antiplatelet drugs.

关 键 词：气动管道传输系统 凝血功能 血小板功能 血小板聚集率 分析前质量控制 

分 类 号：R446.11[医药卫生—诊断学]