祛风除湿方对特应性皮炎小鼠炎性反应和皮肤屏障功能的影响及其作用机制  

Effect of Qufeng Chushi Prescription on inflammatory reaction and skin barrier function in atopic dermatitis mice and its mechanism

作　　者：江雪[1] 杨富金[1] 钱思宇[1] 刘德明 史丙俊[1] JIANG Xue;YANG Fujin;QIAN Siyu;LIU Deming;SHI Bingjun(Department of Dermatology,Chongqing Traditional Chinese Medicine Hospital/Chongqing First People′s Hospital,Chongqing 400011,China)

机构地区：[1]重庆市中医院/重庆市第一人民医院皮肤科,重庆400011

出　　处：《国际检验医学杂志》2020年第18期2208-2213,共6页International Journal of Laboratory Medicine

基　　金：重庆市自然科学基金项目(cstc2015jcyjA10126,cstc2018jcyAX0759);重庆市卫生和计划生育委员会基金资助课题(ZY201802142);成都中医药大学杏林医者项目(YYZX2019058)。

摘　　要：目的通过观察祛风除湿方对二硝基氯苯(DNCB)诱导的特应性皮炎(AD)小鼠炎性反应和皮肤屏障功能的影响,探讨其治疗AD的相关机制。方法将雌性Balb/c小鼠20只随机分为对照组、模型组、地塞米松组和祛风除湿方组,每组5只。除对照组外,其余3组均以DNCB致敏耳部及背部造模。祛风除湿方组给予7.83 g/(kg·d)祛风除湿方灌胃;地塞米松组给予0.59 mg/(kg·d)地塞米松悬液灌胃。比较4组小鼠造模区域的经皮水分流失(TWEL)情况及搔抓次数;比较4组小鼠皮肤组织的典型病理改变;比较4组小鼠皮肤组织中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、凋亡相关的半胱氨酸肽酶14(Caspase-14),血浆IL-4、IL-6、免疫球蛋白E(IgE)、干扰素-γ(IFN-γ),脾组织中IL-17A、维甲酸相关孤核受体γt(RORγt)、转化生长因子-β(TGF-β)、叉状头/翅膀状螺旋转录因子(Foxp3)的表达情况。结果与模型组比较,地塞米松组与祛风除湿方组TWEL明显降低(P<0.05)。与模型组比较,祛风除湿方组TNF-α、IL-1β表达水平明显降低(P<0.05),地塞米松组TNF-α表达水平明显降低(P<0.05),对照组、地塞米松组和祛风除湿方组Caspase-14表达水平明显升高(P<0.05)。与模型组比较,地塞米松组和祛风除湿方组IgE、IL-4、IL-6表达水平明显降低(P<0.05),IFN-γ表达水平明显升高(P<0.05)。与模型组比较,地塞米松组和祛风除湿方组IL-17A、RORγt表达水平明显升高(P<0.05),Foxp3、TGF-β表达水平明显降低(P<0.05)。结论祛风除湿方治疗AD的效果较好,能有效减轻患处皮肤水分流失,减少瘙痒,修复皮肤屏障,其作用机制可能与调节免疫、控制炎性反应等有关。Objective To observe the effect of Qufeng Chushi Prescription on the inflammatory reaction and skin barrier function of mice with atopic dermatitis(AD)induced by dinitrochlorobenzene(DNCB),and to explore the related mechanism of Qufeng Chushi Prescription in the treatment of AD.Methods Twenty female Balb/c mice were randomly divided into control group,model group,dexamethasone group and Qufeng Chushi Prescription group,with 5 mice in each group.Except for the control group,the other 3 groups were modeled with DNCB sensitized ears and back.The Qufeng Chushi Prescription group was given 7.83 g/(kg·d)Qufeng Chushi Prescription by gavage,and the dexamethasone group was given 0.59 mg/(kg·d)dexamethasone suspension by gavage.The transdermal water loss(TWEL)and scratching times of the 4 groups were compared.The typical pathological changes of skin tissues of the 4 groups were compared.The expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand apoptosis-related cysteine peptidase 14(Caspase-14)in skin tissues,IL-4,IL-6,immunoglobulin E(IgE)and interferon-γ(IFN-γ)in plasma,and IL-17A,retinoic acid-related orphan nuclear receptorγt(RORγt),transforming growth factor-β(TGF-β),fork-shaped head/wing-shaped spiral transcription factor(Foxp3)in spleen tissues of the 4 groups were compared.Results Compared with the model group,the TWEL in the dexamethasone group and the Qufeng Chushi Prescription group significantly reduced(P<0.05).Compared with the model group,the expression levels of TNF-αand IL-1βin the Qufeng Chushi Prescription group significantly reduced(P<0.05),and the expression level of TNF-αin the dexamethasone group significantly reduced(P<0.05),the expression levels of Caspase-14 in the control goup,dexamethasone group and the Qufeng Chushi Prescription group significantly increased(P<0.05).Compared with the model group,the expression levels of IgE,IL-4 and IL-6 in the dexamethasone group and the Qufeng Chushi Prescription group significantly reduced(P<0.05),and the expression level of IFN-γ

关 键 词：特应性皮炎 祛风除湿方 免疫调节 皮肤屏障 

分 类 号：R275.9[医药卫生—中西医结合]