桃SPL基因家族成员鉴定及其基因表达与果实发育的相关性分析  

Identification of SPL Gene Family Members and Correlation Analysis Between Gene Expression and Fruit Development in Peach(Prunus persica)

作　　者：汪先菊 张绍宇[1] 徐泽 石佩[1] 康同洋 李琴 赵彩平[1] WANG Xian-Ju;ZHANG Shao-Yu;XU Ze;SHI Pei;KANG Tong-Yang;LI Qin;ZHAO Cai-Ping(College of Horticulture,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学园艺学院,杨凌712100

出　　处：《农业生物技术学报》2020年第9期1521-1534,共14页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31572079);陕西省重点研发计划(2018NY-048)。

摘　　要：SQUAMOSA启动子结合蛋白(SQUAMOSA promoter-binding protein like,SPL/SBP)基因家族在植物的胚胎、芽、叶片、花、果实等器官生长发育过程中起着重要作用。本研究鉴定了桃中SPL基因家族成员,并分析了其在不同组织及果实发育过程中的表达特性。结果表明,桃中共有25个PrupeSPL基因,其中除了PrupeSPL16缺少Zn-2结构外,其他所有PrupeSPL基因均含有1个SBP保守结构域,2个锌指结构和1个核定位信号。SPL基因家族的大部分成员在不同组织中均有表达,且多数成员在幼果中表达量较高。PrupeSPL1,3,4,7,8,10,13,14,16~21,24,25随着果实生长发育表达量显著下调,揭示这些成员可能参与了桃幼果发育的调控;而PrupeSPL1~4,7,8,10,16,17,19,21随果实成熟软化呈显著上调表达趋势,表明这些成员可能与桃果实发育后期的成熟软化及衰老相关。本研究对桃PrupeSPL基因家族进行了系统的分析,初步筛选了可能参与调控桃果实生长发育和成熟软化进程的PrupeSPL基因成员,为进一步研究SPL基因在桃果实发育过程中的功能特性提供了基础资料。SQUAMOSA promoter-binding protein like(SPL)gene family plays an important role in growth and development of embryos,buds,leaves,flowers,fruits and other organs in plants.The members of the SPL gene family in peach were identified and their expression characteristics in different tissues and fruit development processes were analyzed in this study.The results showed that 25 PrupeSPL gene family members were identified from the peach genome.Except that PrupeSPL16 lacked the Zn-2 structure,all other PrupeSPL genes contain an SBP conserved domain,two zinc finger structures and a nuclear localization signal.Most members of the SPL gene family were expressed in different tissues,and most members had higher expression in young fruit.With the fruit growth and development,the expression level of PrupeSPL1,3,4,7,8,10,13,14,16~21,24,25 significantly decreased,revealing that these members might be involved in the regulation of peach young fruit.During the fruit ripening and softening,the expression of PrupeSPL1~4,7,8,10,16,17,19,21 showed a significant up-regulation trend,indicating that these members might be related to peach fruit ripening,softening and senescence.In this study,the PrupeSPL gene family was systematically analyzed in peach,and the PrupeSPL members that might be involved in regulating the growth,ripening and softening process of peach fruit were screened.This study provides basic data for further elucidating the function of SPL gene during peach fruit development.

关 键 词：桃 桃SQUAMOSA启动子结合蛋白(PrupeSPL) 果实发育 表达模式 成熟软化 

分 类 号：S662.1[农业科学—果树学]