鸭不同羽色毛囊组织差异表达基因转录组测序分析  

Transcriptome Sequencing Analysis of Differentially Expressed Genes Associated with Differential Color Feather Bulbs in Ducks(Anas platyrhynchos)

作　　者：蒋兵兵 黄曼曼 白天 曾涛[2] 陈黎[2] 沈军达[2] 陶争荣[2] 周玮 刘国发 卢立志[2] 李海英[1] JIANG Bing-Bing;HUANG Man-Man;BAI Tian;ZENG Tao;CHEN Li;SHEN Jun-Da;TAO Zheng-Rong;ZHOU Wei;LIU Guo-Fa;LU Li-Zhi;LI Hai-Ying(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Institude of Animal Science and Veterinary,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Jiangsu Guiliu Animal Husbandry Group Co.,Ltd.,Xuzhou 221600,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]浙江省农业科学院畜牧兽医研究所,杭州310021 [3]江苏桂柳牧业集团有限公司,徐州221600

出　　处：《农业生物技术学报》2020年第9期1623-1634,共12页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划项目(2015BAD03B06);国家水禽产业技术体系项目(CARS-3-6);浙江省农业(畜禽)新品种选育重大科技专项(2016C02054-12)。

摘　　要：羽色作为鸭(Anas platyrhynchos)的重要经济性状和品种特征,有着重要的研究意义,但其遗传机制尚不明确。为研究鸭不同羽色毛囊组织转录组差异、丰富鸭毛囊转录组数据信息,本研究选用'连城白鸭'与'樱桃谷鸭'杂交F1代花色羽鸭4羽(取每羽鸭背部黑色羽毛和腹部白色羽毛,共8个样品),利用二代测序分析了同1个体不同羽色毛囊组织的转录组特征。本研究共获得449067116个高质量数据,总碱基数目为67.36 Gb,每个样本均获得了7.4 Gb以上的碱基,8个样品平均GC含量为52%,碱基测序错误率低于1%(Q20)的数据均在96.73%以上,其中平均73.94%的高质量数据可比对到鸭参考基因组上。对两种羽色毛囊组织转录组进行比较,发现有231个差异表达基因,其中相较于白羽毛囊组织而言,黑色毛囊组织中上调基因有175个,下调基因有56个,包括与黑色素合成过程相关的基因,如酪氨酸相关蛋白1基因(tyrosinase-related protein1,TYRP1)、酪氨酸酶基因(tyrosinase,TYR)、干细胞因子受体基因(stem cell factor receptor,KIT)、溶质载体45家族第2成员基因(solute carrier family 45 member 2,SLC45A2)、黑素亲和素基因(melanophilin,MLPH)、黑色素A基因(melan-A,MLANA)、多巴色素异构酶基因(dopachrome tautomerase,DCT/TYRP2)、瞬时受体势M亚基1基因(transient receptor potential cation channel subfamily M member 1,TRPM1)、眼皮肤白化病Ⅱ型基因(oculocutaneous albinism typeⅡ,OCA2)、SOX基因家族第10成员(SOX gene family member 10,SOX10)等,且这些基因在黑色羽毛毛囊中表达量要显著高于在白色毛囊中表达量。基因本体(Gene Ontology,GO)分析发现19个差异基因显著富集于8个GO term,包括G蛋白偶联受体信号通路、跨膜受体活性、分子传感器活性等;KEGG通路分析表明3个通路显著富集,包括神经活性配体-受体相互作用、酪氨酸代谢和黑色素合成通路,其中黑色素合成通路和酪氨酸代谢通路与鸭羽As an important economic trait and breed characteristic of duck(Anas platyrhynchos),plumage color has important research significance,but its genetic mechanism is still unclear.In order to study the differences in the transcriptome of white and black feather bulbs and offer new information related to gene expression profiles in black and white feather bulbs in ducks,4 F1 white-black plumage crossbred ducks of'Liancheng white duck'and'Cherry Valley duck'were selected to analyze the transcriptome characteristics of white and black feather bulbs in the same individual in this study.457208376 raw cleans were obtained in 8 samples.After removing sequencing adaptors and the low-quality reads,449067116 clean reads were obtained,accounting for 98.23%of the raw reads.The total base number of which was 67.36 Gb,and each sample obtained bases above 7.4 Gb,with the average GC content of 8 samples being 52%,the Q20 base percentage at 96.73%and above,and average 73.94%being successfully mapped to the duck genome.70.85%of the mapped reads were uniquely aligned to the duck genome.The expression genes were screened using FPKM(expected number of fragments per kilobase of transcript sequence per millions base pairs sequenced)≧1 as the threshold.It was found that 14296 genes were expressed in one sample at least.231 significantly differentially expressed genes were identified(P≦0.05,|log2Fold Change|≧1)between white and black feather bulbs,including 56 down-regulated and 175 up-regulated in black feather bulbs when compared with the white feather bulbs.Several genes related to melanogenesis were found in these differentially expressed genes,such as tyrosinase-related protein1(TYRP1),tyrosinase(TYR),stem cell factor receptor(KIT),melanophilin(MLPH),dopachrome tautomerase(DCT/TYRP2),melan-A(MLANA),transient receptor potential cation channel subfamily M member 1(TRPM1),oculocutaneous albinism typeⅡ(OCA2),SOX gene family member 10(SOX10)and so on,and the expression of these genes in black feather bulbs was significantly higher t

关 键 词：鸭 羽色 毛囊 差异表达基因 黑色素 转录组 

分 类 号：S834[农业科学—畜牧学]