水稻单基因系品种IRBL9-W抗瘟初期基因表达谱分析  

Analysis of Gene Expression Profiles of Rice(Oryza sativa)Monogenic Line of IRBL9-W During Its Early Stage of Resistance to Magnaporthe oryzae

作　　者：姜兆远[1,2] 任金平 李莉[1,2] 刘晓梅 朱峰[1,2] 王继春 孙辉[1,2] JIANG Zhao-Yuan;REN Jin-Ping;LI Li;LIU Xiao-Mei;ZHU Feng;WANG Ji-Chun;SUN Hui(Institute of Plant Protection,Jilin Academy of Agricultural Sciences,Changchun 130033,China;Key Laboratory of Integrated Pest Management on Crops in Northeast,Ministry of Agriculture,Gongzhuling136100,China)

机构地区：[1]吉林省农业科学院植物保护研究所,长春130033 [2]农业部东北作物有害生物综合治理重点实验室,公主岭136100

出　　处：《农业生物技术学报》2020年第9期1662-1674,共13页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划项目(2017YFD0300606)。

摘　　要：水稻(Oryza sativa)单基因系品种’IRBL9-W’(含抗病基因Pi9)对吉林省采集的大部分稻瘟菌(Magnaporthe oryzae)菌株表现抗性,说明含无毒基因(avirulence, AVR)的菌株在吉林省广泛存在。为了探究Pi9与无毒基因互作(AVR-Pi9)初期水稻基因表达谱及其抗病相关信号通路中基因表达情况,本研究将稻瘟菌菌株C2 (AVR-Pi9)接种于水稻单基因系’IRBL9-W’(Pi9),通过高通量RNA-Seq技术对接种36 h后的水稻叶片和未接种水稻叶片进行转录组表达谱分析,共获得差异表达基因2 311个。基因功能注释和代谢通路分析显示转录调控因子中4个亮氨酸拉链蛋白基因中1个上调表达,1个锌指蛋白基因上调表达,而WRKY蛋白基因全部下调表达;真菌细胞壁降解酶中10个几丁质酶基因中2个上调表达,2个β-1,3葡聚糖酶基因全部下调表达;植物-病原体互作信号通路中1个病程相关蛋白1基因(pathogenesisrelated protein 1, PR1)上调表达,其他基因下调表达;类黄酮化合物生物合成信号通路中的差异表达基因全部上调表达;苯丙素类化合物生物合成信号通路中9个基因上调表达1个基因下调表达。3个信号通路中差异表达基因产物,主要参与活性氧的产生、过敏性坏死反应及抗氧化过程。信号通路中差异基因产物对应化合物的功能包括抗氧化、抑菌及木质化作用。本研究为进一步了解水稻抗稻瘟病机制提供理论依据。The rice(Oryza sativa) monogenic line of ’IRBL9-W’(containing resistance gene(Pi9)) was resistant to most of the Magnaporthe grisea isolates collected in Jilin province, indicating that the isolates contain the avirulence gene(AVR) widely spread in Jilin Providence. To investigate the gene expression profiles of the rice during the early interaction between Pi9 and avirulence gene(AVR-Pi9), the rice monogenic line of ’IRBL9-W’(Pi9) inoculated with Magnaporthe grisea isolate C2(AVR-Pi9) in this study.The RNA-seq technology was used to analyze the expression profiles of the inoculated and uninoculated rice leaves of ’IRBL9-W’(Pi9) at 36 h after inoculation and a total of 2 311 genes were found to express differently in the two samples. The analyses of GO(Gene Ontology) and KEGG(Kyoto Encyclopedia of Genes and Genomes) pathways showed that 3 of the 4 leucine zipper protein genes were up-regulated, 1 zinc finger protein gene was up-regulated and all the WRKY protein genes were down-regulated among transcriptional regulator genes, 2 chitinase genes were up-regulated among the10 chitinase genes and all the two β-1, 3 glucanase genes were down-regulated among the fungal cell wall degrading enzyme genes, 1 pathogenesisrelated protein 1 gene(PR1) was up-regulated and the other genes were down-regulated in the plant-pathogen interaction pathway, all of the differentially expressed genes were up-regulated in the flavonoid biosynthesis pathway, and 9 genes were up-regulated and 1 gene was down-regulated in the phenylpropanoid biosynthesis pathway. The differentially expressed gene products in the 3 pathways were involved in getting the reactive oxygen species, the hypersensitive response, and the antioxidant process. The functions of the compounds relating to these gene products were antioxidation, antifungal action, and lignification. This study would provide a theory to further understand the mechanism of rice blast resistance.

关 键 词：稻瘟病 基因表达谱 黄酮类化合物 苯丙素类化合物 

分 类 号：S184[农业科学—农业基础科学]