花生Argonaute基因家族全基因组鉴定与表达模式分析  

Genome-wide identification and expression pattern analysis of peanut Argonaute gene family

作　　者：陆晓东 李春娟[1] 张浩[1] 孙全喜[1] 闫彩霞[1] 赵小波[1] 王娟[1] 苑翠玲 单世华[1] LU Xiao-dong;LI Chun-jua;ZHANG Hao;SUN Quan-xi;YAN Cai-xia;ZHAO Xiao-bo;WANG Juan;YUAN Cui-ling;SHAN Shi-hua(Shandong Peanut Research Institute,Qingdao 266100,China)

机构地区：[1]山东省花生研究所,山东青岛266100

出　　处：《中国油料作物学报》2020年第5期767-777,共11页Chinese Journal of Oil Crop Sciences

基　　金：泰山学者特聘专家(ts201712080);山东省现代农业产业技术体系(SDAIT-04-02);山东省农业良种工程(2017LZGC003);山东省农业科学院农业科技创新工程(CXGC2016A01);农业部科研杰出人才及其创新团队培养(13190194)。

摘　　要：AGO蛋白(Argonaute protein)是RNA诱导沉默复合体的关键组分,在植物生长发育中发挥重要作用。花生基因组测序的完成为全基因组水平上分析AGO抗病基因提供了条件。利用AGO蛋白的保守域在花生基因组数据库与NCBI中进行同源比对,鉴定得到花生AGO基因家族所有成员。我们基于生物信息学对AGO蛋白家族的进化关系、理化性质、染色体定位、基因结构、结构域、不同组织中和胁迫下的表达模式等进行分析。结果表明:试验共鉴定得到51个花生AGO基因,包括12个A.duranensis基因,12个A.ipaensis基因以及27个栽培种花生AGO基因。染色体定位分析结果显示这些基因不均匀地分布在花生染色体上,且A.duranensis与A.ipaensis基因组上有10对成员存在较为明显的同源关系。表达模式分析表明AdAGO2、AiAGO4、AdAGO3、AiAGO7、AdAGO8、AiAGO8基因在花生22个组织中整体表达量偏高;而花生茎尖(Shoot Tip)与雄蕊(Stamens)中AGO基因家族呈现较高表达量。本研究结果为揭示AGO蛋白功能和发掘花生的抗逆育种靶向基因资源提供了一定的理论依据。AGO(Argonaute)protein is a core component of RNA induced silencing complex(RISC)and plays an important role in plant growth and development.The completion of peanut genome sequencing provides the condition for analyzing the AGO gene family on the whole genome.The conserved domain of AGO protein was used to obtain the members of the peanut AGO gene family which was based on the Peanut base database and NCBI.The phylogenetic trees,physicochemical properties,chromosomal distribution,genes structure,domain structure,and gene expression patterns in 22 tissues and under stresses were analyzed.The results showed that 51 members of the peanut AGO genes including 12 of the A.duranensis genomes,12 of the A.ipaensis genomes and 27 of the peanut cultivars were identified.Chromosomal location analysis showed that these members were unevenly distributed on the peanut chromosomes,and 10 pairs of AGO members from the A.duranensis and A.ipaensis genomes had a significant homology relationship.The expression pattern analysis shown that AdAGO2,AiAGO4,AdAGO3,AiAGO7,AdAGO8,and AiAGO8 had high-level expression in 22 tissues of peanut;and the AGO gene family shown higher expression in peanut shoot tip and stamens than other tissues.This study provided some theoretical evidence for further revealing the function of AGO protein,and exploring targeted gene resources related to stress-resistant breeding in peanut.

关 键 词：AGO蛋白 花生 表达模式分析 

分 类 号：S565.2[农业科学—作物学]