聚-γ-谷氨酸对柑橘苗木性状和GABA的影响  

Effects of poly-γ-glutamic acid on seedlings trait and GABA of citrus

作　　者：宗琪[1] 张生才 范国泰 孙萌 姜玲 ZONG Qi;ZHANG Shengcai;FAN Guotai;SUN Meng;JIANG Ling(State key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China;National Modern Industrial Technology System,Fujian Province Yongchun County Tangerine Comprehensive Experiment Station,Yongchun 362600,China;Ministry of Education Key Laboratory of Plant Biology,Huazhong Agricultural University/Ministry of Agriculture and Rural Affairs Key Laboratory of Horticultural crop Biology and Germplasm Creation in Central China,Wuhan 430070,China)

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070 [2]国家现代产业技术体系福建永春芦柑综合试验站,永春362600 [3]华中农业大学园艺林学学院/国家果树脱毒种质资源室内保存中心/农业农村部华中地区园艺作物生物学与种质创制重点实验室,武汉430070

出　　处：《华中农业大学学报》2020年第5期108-114,共7页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目(2018YFD0201500);国家自然科学基金项目(31872064)。

摘　　要：通过株高、冠径及酶活的测定研究聚-γ-谷氨酸(PGA)水溶液灌根对柑橘苗木个体性状和叶片中抗性相关酶的影响,并采用亲水相互作用色谱(HILIC LC-MS/MS)分析了PGA灌根前后叶片中200个靶向代谢物含量的变化。结果表明:PGA处理能促进苗木株高、冠径和嫁接苗分枝数的增加;PGA处理后,柑橘叶片中超氧化物歧化酶(SOD,抗氧化物保护酶)、过氧化物酶(POD,参与过氧化产物的清理)和多酚氧化酶(PPO,酚类物质代谢的参与酶)的活性增加;PGA处理后,柑橘叶片中L-谷氨酸含量下降73.2%倍,而γ-氨基丁酸(GABA)含量增加了1.6倍,N6-甲基腺嘌呤含量提高2.3倍。靶向代谢物分析获得109个代谢物的定量结果,其中,13种代谢物对照与处理的差异达到显著水平。The effects of poly-γ-glutamic acid(PGA)aqueous solution irrigation on seedlings trait and resistance-related enzymes in leaves of citrus were determined by measuring plant height,crown diameter and enzyme activity.HILIC LC-MS/MS was used to analyze the changes of 200 target metabolites in leaves after irrigation of PGA.The results showed that PGA treatment increased the height,crown diameter and branch number of seedlings.The activities of superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO)in citrus leaves were increased after PGA treatment.The content of L-glutamate in citrus leaves decreased by 73.2%,while the content ofγ-aminobutyric acid(GABA)increased by 1.6 times.The content of N6-methyl adenine increased by 2.3 times.The results of quantities of 109 metabolites analyzed by targeted metabolite showed that the difference of 13 metabolites between control and treatment was significant.

关 键 词：聚-γ-谷氨酸 柑橘苗木 靶向代谢物 Γ-氨基丁酸 抗性相关酶 良种繁育 

分 类 号：S144[农业科学—肥料学] S666.304.3[农业科学—农业基础科学]