初始阻力运动对男性骨骼肌mRNA表达谱的影响及其作用机制  

Effect of initial resistance exercise on mRNA profiles of skeletal muscles of males and the associated mechanisms

作　　者：包桂荣[1] 孙锋[1] BAO Guirong;SUN Feng(Teaching and Research Section of Public Sports,Sports Science College,Nantong University,Nantong 226019,China)

机构地区：[1]南通大学体育科学学院公共体育教研室,江苏南通226019

出　　处：《中国医科大学学报》2020年第9期835-840,共6页Journal of China Medical University

基　　金：江苏省教育科学“十三五”规划(B-a/2016/01/045)。

摘　　要：目的探讨初始阻力运动不同阶段对健康男性骨骼肌mRNA表达谱的影响及其相关机制。方法从美国国家生物技术信息中心(NCBI)人类基因表达汇编(GEO)数据库下载骨骼肌基因表达数据集GSE45426。对照组和阻力运动组分别进行差异表达基因(DEG)分析,明确入组时,1、2个周期阻力运动后骨骼肌的DEG,通过基因本体分析和基因集富集分析探讨其参与的生物过程以及潜在的机制。结果未进行过阻力运动的健康成年男性1个周期急性阻力运动后,骨骼肌细胞内的mRNA表达谱与入组时比较无显著差异。第2个周期运动后,骨骼肌细胞中可见大量DEG,表达上调的DEG参与了促进炎症反应、上皮-间质转化、胶原纤维组织形成与代谢等生物过程;CXCL9、CXCL11、CXCL10、IL4R和IRF1等基因在炎症相关通路中发挥关键作用。结论健康男性进行初始阻力运动1个周期不能引起基因组学改变;而2个周期可促进骨骼肌发生与炎症反应、胶原纤维组织形成、细胞增生等相关的基因组学改变。Objective To evaluate the effect of the distinct stage of resistance exercise(RE)on the mRNA profiles of skeletal muscle cells in healthy RE naive males and to further elucidate the associated mechanisms.Methods The mRNA data on skeletal muscles under the accession code GSE45426 were retrieved from the gene expression omnibus database of the National Center for Biotechnology Information.Differential expression genes(DEG)at baseline and after each of the two sessions of RE were identified in the control and the RE training groups,respectively.Further gene ontology assays and gene set enrichment analysis were performed to identify the biological processes and the potential mechanisms that the DEG were associated with.Results In the healthy RE naive adult males,there was no significant difference between the DEG upregulated in response to one session of exercise and no exercise.However,two sessions of exercise led to the upregulation of multiple DEG in the skeletal muscle cells,and the DEG were associated with various biological processes,such as inflammation,epithelial-mesenchymal transition,collagen fibril organization,and catabolic processes,with CXCL9,CXCL11,CXCL10,IL4R,and IRF1 as the common leading-edge genes in multiple inflammation-related gene sets.Conclusion While a single session of exercise did not significantly alter the mRNA profile in the skeletal muscles of healthy RE naive males,two subsequent sessions of exercise induced multiple alterations in the expression pattern of genes associated with inflammation,collagen reorganization,and cellular proliferation of skeletal muscles.

关 键 词：初始阻力运动 男性 骨骼肌 差异表达基因 

分 类 号：R455[医药卫生—运动医学]