SULT4A1基因4个位点在中国北方汉族群体中的分布及法医学意义  

Distribution and forensic significance of four loci of SULT4A1 in Han population of northern China

作　　者：吴军胜 丁梅[1] 雍梦馨 赵梦阳 邢佳鑫[1] 王保捷[1] WU Junsheng;DING Mei;YONG Mengxin;ZHAO Mengyang;XING Jiaxin;WANG Baojie(Department of Forensic Genetics,School of Forensic Medicine,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学法医学院法医物证教研室,沈阳110122

出　　处：《中国医科大学学报》2020年第9期854-857,861,共5页Journal of China Medical University

摘　　要：目的调查SULT4A1基因rs549267654、rs2285162、rs963263和ss2137544034位点在中国北方汉族群体的多态性分布,评价其法医学应用价值。方法采用Sanger测序及PCR-RFLP技术对226例中国北方汉族健康无关个体样品进行检测,应用Haploview4.2进行数据的统计分析。结果rs549267654、rs2285162和rs963263位点的基因型分布符合Hardy-Weinberg平衡,个人识别力分别为0.649、0.619和0.647,非父排除率分别为0.187、0.177和0.187。同时,发现1个新的6碱基插入/缺失位点ss2137544034。结论rs549267654、rs2285162和rs963263位点在中国北方汉族人群中具有较好的遗传多态性,可用于法医学个人识别与亲权鉴定。Objective To investigate the distribution of polymorphisms in the rs549267654,rs2285162,rs963263,and ss2137544034 loci of the SULT4A1 gene in the Han population of northern China and to evaluate their application value in forensic medicine.Methods Sanger sequencing and PCR-RFLP were used to detect polymorphisms in SULT4A1 in 226 healthy individuals from northern China.The data were analyzed using Haploview 4.2.Results The genotype distributions of rs549267654,rs2285162,and rs963263 were in accordance with Hardy-Weinberg equilibrium;their discrimination powers were 0.649,0.619,and 0.647,respectively,and the probabilities of paternity were 0.187,0.177,and 0.187,respectively.Concomitantly,a new 6-base insertion/deletion site,ss2137544034,was identified.Conclusion rs549267654,rs2285162,and rs963263 exhibit high genetic polymorphism in the Han population of northern China and can be used for forensic personal identification as well as paternity identification.

关 键 词：法医学 多态性 单核苷酸多态性 SULT4A1 汉族 

分 类 号：D919[医药卫生—法医学]