超重现毛细管电泳的理论与例证  

Super stable capillary electrophoresis:theory and examples

作　　者：陈义 CHEN Yi(Huaiyin Institute of Technology,Huaiyin 223001,China;CAS Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China;Beijing National Center for Molecular Sciences,Beijing 100190,China)

机构地区：[1]淮阴工学院,江苏淮阴223001 [2]中国科学院化学研究所,中国科学院活体分析化学重点实验室,北京100190 [3]中国科学院大学,北京100049 [4]北京分子科学国家实验室,北京100190

出　　处：《色谱》2020年第10期1217-1223,共7页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(21727809);中国科学院项目(QYZDJ-SSW-SLH034)。

摘　　要：毛细管电泳经常遭受结果不稳定、不重现的困扰。该文从理论上推导了一些新模式并以例证说明,这些模式能在一定条件下抵抗条件变化,获得超重现电泳谱图。它们是加权淌度谱、迁移电量谱、电荷密度谱、摩尔电荷密度谱、扩散系数谱、液相质量谱,以及它们各自的比例谱等,其中前4种为实时测量模式,其余的为实验后模式。这些模式需要发展新的仪器或算法,但都有发展潜力,值得深入研究。Capillary electrophoresis is often plagued by unstable and unrepeatable results.In this paper,some novel modes are derived theoretically and illustrated by separation examples,as expected these modes can resist the change of some conditions under certain prerequisites and obtain super stable electropherograms.They are weighted mobility spectrometry,migrated charge spectrometry,charge density spectrometry,molar charge density spectrometry,diffusion coefficient spectrometry,liquid-phase mass spectrometry,and their ratio spectrometry.The first four are real-time measurement modes while the others are post-experimental modes.These modes need to develop new devices and algorithm,but they are all highly promising and worth of further exploitation.

关 键 词：毛细管电泳 非时间测量 超重现模式 理论推导 例证 

分 类 号：O658[理学—分析化学]