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作 者:黄青松[1] 胡纯红[1] HUANG Qingsong;HU Chunhong(Chengdu Second People's Hospital,Chengdu,610015,China)
出 处:《医学分子生物学杂志》2020年第4期318-324,共7页Journal of Medical Molecular Biology
基 金:四川省卫计委课题(No.17PJ163)。
摘 要:目的探索三七皂苷R1对鼻咽癌细胞株CNE1恶性生物学行为的影响。方法将CNE1细胞随机分为4组,对照组用含正常对照血清的DMEM培养基培养,分别在其余3组细胞的血清DMEM培养基中加入1、2.5、5μmol/L木犀草素继续培养,CCK8检测各组细胞的存活率;BRDU染色检测细胞增殖状况;流式细胞技术检测细胞凋亡;Transwell实验观察细胞侵袭的情况;划痕实验检测细胞迁移能力;免疫印迹法检测线粒体通路蛋白Caspase-3、Caspase-9、Bax、Bcl-2、c-Myc的蛋白表达。结果三七皂苷R1浓度超过10μmol/L时,HNE1细胞的存活率显著降低(P<0.05)。与对照组比较,2.5μmol/L和5μmol/L三七皂苷R1两个处理组阳性细胞数显著减少(P<0.05);HNE1细胞凋亡率显著升高(P<0.05);划痕闭合率显著降低(P<0.05)。与对照组比较,1、2.5、5μmol/L三七皂苷R13个处理组的侵袭细胞数显著降低(P<0.05)。与对照组比较,5μmol/L三七皂苷R1组Caspase-3,Caspase-9和Bax/Bcl-2蛋白表达水平均显著升高(P<0.05);c-Myc蛋白表达水平显著降低(P<0.05)。结论三七皂苷R1通过抑制线粒体通路相关蛋白表达,促进CNE1细胞凋亡,以及抑制CNE1细胞的增殖、侵袭、迁移,从而影响鼻咽癌细胞株CNE1恶性生物学行为。Objective Explored the effet of Notoginsenoside R1 on the malignant biological behavior of nasopharyngeal carcinoma cell line CNE1.Methods CNE1 cells were randomly divided into four groups.The control group was cultured in DMEM medium containing normal control serum,and the remaining 3 groups were cultured with 1,2.5 and 5μmol/L luteolin respectively.Cell pro-liferation was detected by BRDU staining.Cell apoptosis was detected by flow cytometry.Transwell experiment observed cell invasion.The ability of cell migration was measured by scratch test.Immunoblotting was used to detect the protein expression of mitochondrial pathway:caspase-3,caspase-9,Bax,Bcl-2 and c-Myc.Results When the concentration of Notoginsenoside R1 excee-ded 10μmolL,the survival rate of HNE1 cells decreased significantly(P<0.05).Compared with the control group,the number of positive cells in the two treatment groups of 2.5μmol/L and 5μumol/L notoginsenoside R1 decreased significantly(P<0.05);the apoptosis rate of HNE1 cells increased significantly(P<0.05);the scratch closure rate decreased significantly(P<0.05)Compared with the control group,the number of invasive cells in the three treatment groups of 1,2.5 and 5μumol/L notoginsenoside R1 decreased significantly(P<0.05).Compared with the control group,the expression levels of Caspase-3,Caspase-9 and Bax/Bcl-2 in the 5μmol/L notoginsenoside R1 group decreased significantly(P<0.05);the expression level of c-Myc protein decreased significantly(P<0.05).Conclusion Notoginsenoside R1 can promote apoptosis of CNE1 cells by inhibiting the expression of mitochondrial pathway-related proteins,and inhibit the proliferation,invasion and migration of CNE1 cells,thus affecting the malignant biological behavior of nasopharyngeal carcinoma cell line CNE1.
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