非洲菊单倍体移栽苗培育的关键技术  

Propagation of Transplanted Haploid Plants of Gerbera hybrida via Tissue Culture

作　　者：单芹丽[1,2] 汪国鲜 吴旻[1] 吴丽芳 方洪兵[1,2] 李绅崇 杨春梅 SHAN Qinli;WANG Guoxian;WU Min;WU Lifang;FANG Hongbing;LI Shenchong;YANG Chunmei(Flower Research Institute,Yunnan Academy of Agricultural Sciences,National Engineering Research Center for Ornamental Horticulture,Kunming,Yunnan 650205,China;Yuxi Yunxing Biotechnology Co.,Ltd.,Yuxi,Yunnan 652604,China)

机构地区：[1]云南省农业科学院花卉研究所/国家观赏园艺工程技术研究中心,昆明650205 [2]玉溪云星生物科技有限公司,云南玉溪652604

出　　处：《热带生物学报》2020年第3期341-346,共6页Journal of Tropical Biology

基　　金：云南省技术创新人才培养对象项目(2018HB117);云南省科技厅重点研发项目(2018BB010)。

摘　　要：以非洲菊(Gerbera hybrida)单倍体组培繁殖苗为材料,研究培养基、炼苗时间和基质对非洲菊单倍体生根和移栽的影响。结果表明:生根前的增殖培养基对后期的生根培养影响显著,增殖培养基为MS改良培养液+KT 0.15 mg·L^−1,生根培养基为1/2MS+IBA 0.6 mg·L^−1+NAA 0.1 mg·L^−1,是生根苗培育的最佳培养基组合。室内培养20 d后,移入大棚炼苗4 d,再在V腐植土∶V红土∶V珍珠岩=4∶1∶0.5的混合基质中培育的移栽苗,其炼苗成活率和移栽成活率最高。Gerbera hybrida haploids were propagated via tissue culture to observe the effects of culture medium,domestication and substrates on their rooting and transplanting success.The results showed that proliferation medium before rooting of tissue cultured plants of G.hybrida haploid had a significant effect on rooting culture.The optimum combination of rooting medium was an improved MS medium+KT 0.15 mg·L^−1 for proliferation medium followed by 1/2MS+IBA 0.6 mg·L^−1+NAA 0.1 mg·L^−1 for rooting medium.After 20 days of indoor culture the rooted tissue cultured plants were transferred to the shed for domestication for 4 days and then transferred onto the mixed substrate of humus:laterite:perlite with a volume ratio of 4∶1∶0.5 for further hardening off,based on which the tissue cultured plants were the highest in domestication and transplanting survival rate.

关 键 词：非洲菊 单倍体 生根 组织培养 基质 

分 类 号：S682.11[农业科学—观赏园艺]