桑树PHR家族基因的鉴定及生物信息学分析  

Identification and Bioinformatics Analysis of PHR Family Gene in Morus notabilis

作　　者：韩利红 刘潮 刘学林 刘芸伟 苏娅 Han Lihong;Liu Chao;Liu Xuelin;Liu Yunwei;Su Ya(Center for Yunnan Plateau Biological Resources Protection and Utilization,College of Biological Resource and Food Engineering,Qujing Normal University,Qujing,655011)

机构地区：[1]曲靖师范学院生物资源与食品工程学院,云南高原生物资源保护与利用研究中心,曲靖655011

出　　处：《分子植物育种》2020年第18期5991-5999,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31860005);云南省地方本科高校基础研究联合专项(2017FH001-034);国家级大学生创新创业训练计划项目(201710684012;201810684001;201810684010)共同资助。

摘　　要：磷是植物生长发育的主要矿质营养元素之一,磷饥饿响应(phosphate starvation response, PHR)基因在调节植物磷素吸收中起着重要作用。鉴定桑树磷饥饿响应转录因子家族成员,分析其生物信息学特征,为桑树PHR家族基因的功能研究提供基础。从桑树基因组数据库中获得桑树PHR转录因子序列,利用GSDS、ExPASy、MEGA、MEME、psRNATarget、STRING等软件和网上转录组数据,对桑树PHR家族成员的基因结构、蛋白理化性质、保守基序、系统进化、基因组织表达、调控miRNA和蛋白互作网络进行分析。从桑树基因组中共鉴定出12个PHR基因家族成员,氨基酸数介于256~1 529之间,83.3%的成员属于酸性蛋白,所有成员均为亲水性蛋白。进化分析显示,MnPHR转录因子归为9个聚类组,各聚类组含有1~2个MnPHR成员。外显子数为6、7、8、19的MnPHR编码基因成员分别有7个、3个、1个、1个,同一聚类组中的基因结构类似。发现4个保守性较强的基序,所有MnPHR转录因子均含有基序1~4,聚类组Ⅰ~Ⅳ的MnPHR转录因子缺少基序6。基因组织表达分析发现10个桑树PHR基因在不同组织中有表达,且存在组织特异性,部分基因转录可能受miRNA的调控。MnPHR1的蛋白互作网络分析发现,其主要参与了纤维素合成和转录因子表达调控等生物学过程。桑树PHR家族基因结构和氨基酸序列具有较强的保守性,其组织表达和蛋白互作网络结果表明该家族基因在植物的生长发育和营养吸收过程中发挥作用。本研究结果为深入开展桑树磷吸收和转运相关基因的克隆和功能验证提供了基础。Phospho rus is one of the m ajor mineral nutrients for plant growth and development.Phosphate starvation response(PHR)genes play central roles in regulating plant phosphorus uptake.To identify the members of the phosphate starvation response transcription factor family of mulberry and analyze their bioinformatics characteristics,and to provide a basis for the functional research of the genes of mulberry PHR family.PHR family member sequences were obtained from mulberry genome database.The gene structure,protein physical and chemical properties,conserved motifs,phylogenetic,gene tissue expression,regulation of microRNAs and protein interaction networks of mulberry PHR family members were analyzed by using software such as GSDS,ExPASy,MEGA,MEME,ps RNATarget,STRING and published transc riptome data.A total of 12 PHR gene family members were identified from the mulberry genome,and the number of coding amino acids ranged from 256 to1529,and all of them were hydrophilic proteins.The results showed that MnPHR transcription factors were divided into nine groups,members of each group were one to two.The number of MnPHR coding genes with exons six,seven,eight and nineteen were seven,three,one and one,respectively.Four conservative motifs were found in MnPHR transcription factors,and all MnPHRs contained motifs first to fourth.The MnPHR transcription factors of groupⅠ~Ⅳlacked motifs sixth.Gene expression analysis showed that ten mulberry PHR genes were expressed in different tissues,and showed tissue expression specificity.Transcription of multiple genes was regulated by microRNAs.The protein interaction network analysis of MnPHR1 found that it was mainly involved in biological processes such as cellulose synthesis and transcription factor expression regulation.The PHR family gene structure and amino acid sequence of mulberry were strongly conserved,and the results of tissue expression and protein interaction network showed that the PHR family gene played a role in the growth and development of plants and nutrient absor

关 键 词：桑树(Morus notabilis) 磷饥饿响应转录因子 基因表达 MICRORNA 

分 类 号：S888.2[农业科学—特种经济动物饲养]