水稻短光敏不育基因(rpms4)的遗传分析及初步定位  

Preliminary Mapping and Genetic Analysis of Reverse Photoperiod-sensitive Genic Male Sterility Gene (rpms4) in Rice

作　　者：曹志斌 曾博虹 唐秀英[1] 毛凌华[1] 蔡耀辉[1] 吴晓峰[1] 袁林峰[1] Cao Zhibin;Zeng Bohong;Tang Xiuying;Mao Linghua;Cai Yaohui;Wu Xiaofeng;Yuan Linfeng(Laboratory of Rice National Engineering,Jiangxi Research and Development Center of Super Rice,Jiangxi Academy of Agricultural Sciences,Nanchang,330200)

机构地区：[1]江西省农业科学院,江西省超级水稻研究发展中心,国家水稻工程实验室,南昌330200

出　　处：《分子植物育种》2020年第18期6044-6049,共6页Molecular Plant Breeding

基　　金：江西省农业科学院博士启动基金(20142C13S003);江西省自然科学基金面上项目(20171BAB204020);江西省农业科研协同创新专项项目(JXXTCXFY201902);江西省重点研发计划项目(20171ACF60019)共同资助。

摘　　要：为了深入研究水稻短光敏不育基因,以D38S为母本,华占为父本构建了一个F_2分离群体,对短光敏不育基因控制的遗传规律进行分析。利用集团分离分析法(bulked segregant analysis,BSA)筛选多态性SSR标记,并在多态性标记附近增加标记引物筛选,将获得所有多态性SSR标记对含有248个单株的D38S×华占杂交得到F_2群体进行定位分析。D38S×华占的F_1代植株在南昌早季镜检花粉育性表现正常,自然结实率也正常。对248个F_2群体单株进行育性调查,统计短光可育株与短光不育株分离比,发现短光可育株与短光不育株数分离比符合3:1的分离比,因此可以推测D38S的短光敏不育性状遗传模式符合受1对隐性核基因控制模式。该研究利用集团分离分析法(BSA)从均匀覆盖水稻染色体基因组的3600对SSR中筛选到7对与该不育基因连锁的标记。利用D38S×华占的F_2群体和7对SSR标记,将短光敏不育基因定位于水稻1号染色体,位于标记RM3442与RM6339之间,遗传图距均为1.2 cM,将该短光敏不育新基因命名为rpms4。本研究为进一步精细定位和克隆rpms4提供了科学依据。In order to study the reverse photoperiod-sensitive genic male sterility gene of rice.We choose D38 S as mother parent and Huazhan as father parent to construct a F2 segregation population for genetic pattern analysis of reverse photoperiod-sensitive genic male sterility gene.The bulked segregant analysis(BSA)method is used to screen polymorphic SSR markers.And increasing marker density near the polymorphic markers region.All the polymorphic markers were used for gene mapping analyses in the F2 population(248 plants)from the D38 S×Huazhan.All the F2 plants’fertility phenotypes were detected,and the segregation ratio of fertility phenotypes in the F2 population was consistent with the 3:1 ratio of Mendelian inheritance.This indicated that male sterility was controlled by a recessive gene.Out of 3600 SSRs,7 were linked with the gene.The genetic linkage map suggested that the gene was located on chromosome 1 of the rice genome within markers RM3442 and RM6339,with a 1.2 cM genetic distance to them.This reverse photoperiod-sensitive male sterility gene was designed as rpms4.The mapping results for rpms4 layed a foundation for the fine-mapping and cloning rpms4.

关 键 词：水稻(Oryza sativa) 短光敏基因 rpms4 基因定位 

分 类 号：S511[农业科学—作物学]