干旱诱导AtGSTF14基因DNA去甲基化  

Drought-introduced DNA Demethylation of AtGSTF14 Gene

作　　者：孟大伟[1] 王悦 李沛璇 赵宇威 周瑶 韩玉 郎晨婧 金太成[1] 杨丽萍[1] Meng Dawei;Wang Yue;Li Peixuan;Zhao Yuwei;Zhou Yao;Han Yu;Lang Chenjing;Jin Taicheng;Yang Liping(Key Laboratory for Plant Resources Science and Green Production,The School of Life Science,Jilin Normal University,Siping,136000)

机构地区：[1]吉林师范大学生命科学学院,吉林省植物资源科学与绿色生产重点实验室,四平136000

出　　处：《分子植物育种》2020年第18期6108-6113,共6页Molecular Plant Breeding

基　　金：国家青年科学基金项目(31301-043);吉林省教育厅科研项目(JJKH20191013KJ)共同资助。

摘　　要：DNA甲基化在调控植物基因表达方面起重要作用。干旱、低温和盐胁迫严重地影响了植物的生长发育。然而,DNA甲基化是否在干旱诱导的AtGSTF14基因表达过程中起作用还不清楚。本研究揭示了干旱诱导AtGSTF14基因启动子重复序列的DNA去甲基化,并激活了AtGSTF14基因的表达。本研究采用实时荧光定量PCR技术(quantitative real-time PCR,qRT-PCR)检测目的基因的表达,结果表明干旱处理的拟南芥植物中AtGSTF14基因表达水平显著增加,野生型拟南芥(Col-0)被作为对照。亚硫酸盐测序分析表明与野生型拟南芥中AtGSTF14基因DNA甲基化水平相比,干旱处理的拟南芥植物中AtGSTF14启动子区重复序列的DNA甲基化水平显著降低。本研究为进一步探索非生物胁迫诱导植物基因表达的分子机制奠定了基础。DNA methylation plays an important role in regulation of the gene expression in plants.Drought,low-temperature,and salt stress severely influence the growth and development of plants.However,whether DNA methylation acts in the process of drought-induced gene expression is not very clear.The results revealed that drought-induced DNA demethylation in the promoter repeated sequence of AtGSTF14 gene and activated AtGSTF14 expression.We used Quantitative Real-time PCR(qRT-PCR)to detect the aim gene expression,the results had shown that the expression level of AtGSTF14 gene was significantly increased in drought-treated Arabidopsis plants,and wild-type Arabidopsis(Col-0)was used as a control.Bisulfite sequencing analysis showed that DNA methylation level in the promoter repeats of AtGSTF14 was decreased in drought-treated Arabidopsis plants,compared with that in wild-type Arabidopsis(Col-0).This study provided the basis for further exploring the molecular mechanism of gene expression induced by abiotic stress in plants.

关 键 词：拟南芥(Arabidopsis thaliana) AtGSTF14 干旱诱导 DNA去甲基化 

分 类 号：Q943.2[生物学—植物学]