石蒜光合特性比较及其对温度胁迫的响应  

Comparison of Photosynthetic Characteristics of Lycoris radiata and Their Response to Temperature Stress

作　　者：朱玲 张旭 张姣佼 郑玉红[3] 周坚 Zhu Ling;Zhang Xu;Zhang Jiaojiao;Zheng Yuhong;Zhou Jian(Co-Innovation Center for Sustainable Forestry in Southern China,College of Biology and the Environment,Nanjing Forestry University,Nanjing,210037;Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi,214063;Ornamental Plant Research Center,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden MEM.Sun Yat-Sen),Nanjing,210014)

机构地区：[1]南京林业大学生物与环境学院,南方现代林业协同创新中心,南京210037 [2]江苏省原子医学研究所,江苏省分子核医学重点实验室,无锡214063 [3]江苏省中国科学院植物研究所(南京中山植物园),观赏植物研究中心,南京210014

出　　处：《分子植物育种》2020年第18期6165-6173,共9页Molecular Plant Breeding

基　　金：江苏省林业三新工程项目(LYSX [2014(52),2016(15)]);江苏省高校优势学科建设工程项目(PAPD)共同资助。

摘　　要：为寻找适合林下栽培的石蒜种源,本研究通过比较19个种源石蒜光合参数,筛选出高光效种源,并进一步对该种源温度胁迫的响应机制进行分析。结果表明:在19个地理种源石蒜中,南京、黄山种源各项光合参数均处于优势水平,可作为高光效种源扩大生产。其中,南京种源在高温和低温胁迫下,Pn均降低,高温胁迫下Tr始终高于胁迫初期水平,在低温胁迫下Tr表现为不可逆的降低趋势。石蒜ACBP6基因受温度诱导,高温胁迫下的表达量显著高于低温胁迫下。在两种温度胁迫下,ABA含量均增加,但短期的胁迫中低温诱导的ABA浓度更高。石蒜的ACBP6基因和内源激素ABA可能参与了其对温度胁迫的防御反应。本研究为石蒜适宜种源筛选及温度胁迫响应机制研究提供理论依据。In order to find suitable Lycoris radiata provenances for cultivation under the forest,the photosynthetic parameters of 19 L.radiata provenances were compared to select as high light efficiency germplasm.The response mechanism of temperature stress was further analyzed.The results indicated that among L.radiata of 19 provenances,both genetic resources from Nanjing and Huangshan were at the dominant level in photosynthetic parameters and could be selected as the germplasm with high light efficiency for further propagation.For the germplasm from Nanjing,the value of its Pn parameters decreased in the case of low temperature and high temperature.Under high temperature stress its Tr was higher than at the beginning of stress,but under low temperature ress its Tr showed an irreversible downward trend.The expression of ACBP6 gene of L.radiata was also influstenced by temperature induction.It was a significantly higher expression quantity under high temperature stress than under low temperature stress.Under the two stresses,the content of ABA increased,but under low temperature-inducted ABA was a higher content in short-term stress.The ACBP6 gene and endogenous hormone ABA of L.radiata may be involved in its defen se response to temperature stress.The research provided a theoretical basis for the suitable provenance screening and the temperature stress response mechanism of L.radiata.

关 键 词：石蒜(Lycoris radiata(L'Her.)Herb.) 种源 光合作用 温度胁迫 ACBP6 

分 类 号：S682.29[农业科学—观赏园艺]