Early intervention with Di-Dang Decoction prevents macrovascular fibrosis in diabetic rats by regulating the TGF-β1/Smad signalling pathway  

作　　者：ZHOU Sheng-Nan ZHANG Jian REN Qiu-Yue YAO Rong-Fei LIU Peng CHANG Bai 

机构地区：[1]Nutritional Department,TEDA International Cardiovascular Hospital,Tianjin 300457,China [2]Key Laboratory of Cancer Prevention and Therapy,Tianjin Medical University Cancer Institute and Hospital,Tianjin Medical University,Tianjin 300060,China [3]Graduate School of Tianjin University of Traditional Chinese Medicine,Tianjin 300070,China [4]Key Laboratory of Hormones and Development(Ministry of Health),Tianjin Key Laboratory of Metabolic Diseases,Tianjin Metabolic Diseases Hospital&Tianjin Institute of Endocrinology,Tianjin Medical University,Tianjin 300070,China [5]不详

出　　处：《Chinese Journal of Natural Medicines》2020年第8期612-619,共8页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81473622)。

摘　　要：Macroangiopathy is a complication of Type II Diabetes Mellitus(T2 DM),which is mainly caused by fibrosis of blood vessels.Using T2 DM rat models,we investigated whether the traditional Chinese medicine,Di-Dang Decoction(DDD),exhibited antifibrotic actions on great vessels.T2 DM rats were randomly divided into non-intervention group,early-,middle-,late-stage DDD intervention groups and control groups,including pioglitazone group and aminoguanidine group.After administration of DDD to T2 DM rats at different times,we detected the amount of extracellular matrix(ECM)deposition in the thoracic aorta.The results showed that early-stage intervention with DDD could effectively protect great vessels from ECM deposition.Considering that TGF-β1 is the master regulator of fibrosis,we further validated at the molecular level that,compared to middle-and late-stage intervention with DDD,early-stage intervention with DDD could significantly decrease the expression levels of factors related to the activated TGF-β1/Smad signalling pathway,as well as the expression levels of downstream effectors including CTGF,MMP and TIMP family proteins,which were directly involved in ECM remodelling.Therefore,early-stage intervention with DDD can reduce macrovascular fibrosis and prevent diabetic macroangiopathy.

关 键 词：Di-Dang Decoction MACROANGIOPATHY Fibrosis Diabetes TGF-Β1 CTGF MMP TIMP 

分 类 号：R965[医药卫生—药理学]