腹主动脉缩窄法建立大鼠心衰模型的研究  

Research of the Establishment of Rat Model of Heart Failure by Abdominal Arterial Constriction

作　　者：谭巍 任澎[2] TAN Wei;REN Peng(Department of Cardiology,Xinjiang Autonomous Region People’s Hospital,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区人民医院,乌鲁木齐830000 [2]新疆维吾尔自治区人民医院心内科,乌鲁木齐830000

出　　处：《实验动物科学》2020年第3期19-24,共6页Laboratory Animal Science

基　　金：新疆维吾尔自治区自然科学基金(No.2015211C195)。

摘　　要：目的通过研究不同程度缩窄腹主动脉对Wistar大鼠心脏功能的影响,建立大鼠心衰模型。方法将Wistar大鼠随机分成3组,A组为假手术组,B组缩窄腹主动脉直径至0.45 mm,C组缩窄腹主动脉直径至0.7 mm,术后观察大鼠生存情况、精神状态等一般情况,在4周和8周分别应用超声心动图检测舒张末期室间隔厚度(IVSd)、舒张末期左室后壁厚度(LVPWd)、左室舒张末期内径(LVEDd)、左室收缩末期内径(LVEDs)和射血分数(LVEF),并取标本测量左心室质量指数LVWI、心/体比、肺/体比,评价心功能的变化。结果术后A组存活率为100%,B组存活率为55%,C组存活率为70%;4周时B组IVSD、LVPWd、LVEDd、LVEDs、LVEF与A组和C组均有显著差异(P<0.05),C组与A组相比IVSD、LVPWd、LVEDs有所增加,而LVEDd、EF差异无统计意义(P>0.05)。8周时B、C两组IVSD、LVPWd、LVEDd、LVEDs、LVEF与A组相比差异显著(P<0.05);8周时LVWI、心/体比、肺/体比B组和C组分别与A组相比均有极显著差异(P<0.01),C组与B组相比LVWI、心/体比有显著差异(P<0.05),肺/体比差异极显著(P<0.01)。结论腹主动脉缩窄至直径0.45 mm比缩窄至0.7 mm心衰成模所需时间短,成模效率高,是建立大鼠心衰模型的理想方法。Objective To establish rat model of heart failure by constriction of abdominal aorta,and to compare the effects of abdominal aorta narrowing with different diameters on hemodynamics and pathophysiology in rats.Method Wistar rats were randomly divided into three groups:sham-operated group(group A);narrowed abdominal aorta diameter up to 0.45 mm(group B);narrowed abdominal aorta diameter up to 0.7 mm(group C);observed the survival condition and mental state of the rats after the operation,at 4 and 8 weeks detected the interventricular septal depth(IVSd)、left ventricular posterior walldepth(LVPWd)、left ventricular end diastolic diameter(LVEDd)、left ventricular end systolic diameter(LVEDs)、left ventricular ejection fraction(LVEF)by echocardiography,and left ventricular mass index(LVMI)、heart/body ratio、lung/body ratio were investigated after8 weeks.Result The postoperative survival rate in group A is 100%,in group B is 55%,in group C is 70%;After4 weeks,IVSd,LVPWd,LVEDs,LVEDd and EF in group A were significantly different from those in group B and C(P<0.05).Compared with group A,rats in group C showed increased IVSD、LVPWd and LVEDs,however,LVEDd and EF had no statistically difference(P>0.05).While after 8 weeks,IVSD、LVPWd、LVEDd、LVEDs、LVEF in group B and C both had more significant changes compared with those in group A(P<0.05);at 8 weeks LVMI、heart/body ratio、lung/body ratio were increased markedly in both group B and group C compared with group A(P<0.01),meanwhile compared with group C,LVWI and heart/body ratio were much higher than those in group B(P<0.05),and lung/body ratio increased more obviously(P<0.01).Conclusion Coarctation of abdominal aorta diameter up to 0.45 mm required shorter time,and has more efficient than narrowed up to 0.7 mm in heart failure model establishment,it is an ideal method to establish rat model of heart failure.

关 键 词：心力衰竭 腹主动脉缩窄 大鼠心衰模型 

分 类 号：Q95-33[生物学—动物学]