探究左旋甲状腺素治疗孕期母鼠亚临床甲减的最佳时期  

Optimal Timing of L-Thyroxine Treatment for Subclinical Hypothyroidism in Rats during Pregnancy

作　　者：王鑫 罗才全 李小兰 唐雪 WANG Xin;LUO Caiquan;LI Xiaolan;TANG Xue(Department of Gastroenterology QianJiang Central Hospital of Chongqing Municipality,Chongqing 409000,China;Department of Maternity,QianJiang Central Hospital of Chongqing Municipality,Chongqing 409000,China)

机构地区：[1]重庆市黔江中心医院产科,重庆409000 [2]重庆市黔江中心医院消化内科,重庆409000

出　　处：《实验动物科学》2020年第3期25-30,共6页Laboratory Animal Science

摘　　要：目的分析左旋甲状腺素(L-T4)治疗孕期母鼠亚临床甲减的最佳时期。方法选取150只SPF级Wistar雌鼠按数字表法随机分为6组(n=25):①对照组;②甲减组;③亚甲减组;④亚甲减治疗A组;⑤亚甲减治疗B组;⑥亚甲减治疗C组。采用酶联免疫法测定孕鼠血清促甲状腺激素(TSH)、总甲状腺素(TT4)水平含量;采用Nissl染色法观察大脑皮质和海马形态学变化;采用Morris水迷宫实验法检测仔鼠学习记忆能力。探究L-T4治疗孕期母鼠亚临床甲减最佳时期。结果P3、P7、P40时亚甲减组仔鼠体质量低于对照组(P<0.05),其中P3时甲减组仔鼠体质量低于亚甲减组(P<0.05);P7时甲减组仔鼠体质量显著低于亚甲减治疗A组、B组(P<0.05);P40时甲减组仔鼠体质量低于亚甲减治疗B组、C组(P<0.05),差异有统计学意义。对照组较其他各组仔鼠逃避潜伏时间下降趋势明显,且呈稳定进行性下降。亚甲减组、亚甲减治疗C组与甲减组仔鼠皮质及海马各层神经细胞排列紊乱,且锥体细胞较小。结论亚临床加减可明显影响Wistar雌鼠体质量正常发育,降低后代学习记忆能力,若在E13之前给予L-T4治疗可逆转该损害,改善妊娠不良结局。Objective To investigate the optimal timing of L-thyroxine(L-T4)treatment for subclinical hypothyroidism(SCH)in rats during pregnancy.Method One hundred and fifty SPF Wistar female rats were randomly divided into 6 groups in accordance with the random number table,each with 25 cases,namely,control group,SCH group,hypothyroidism(CH)group,group A,group B and group C.Enzyme-linked immunosorbent assay was used to determine the levels of serum thyroid-stimulating hormone(TSH)and total thyroxine(TT4)in pregnant rats.The Morris water maze test was used to detect the learning and memory ability of rats offspring.Then the optimal timing of the treatment was analyzed.Result At P3,P7 and P40,the weight of offspring of CH group was lower than that of the control group(P<0.05).At P3,the weight of offspring was higher in the SCH group than in the CH group(P<0.05).At P7,the weight of offspring of CH group was lower than that of the group A and group B(P<0.05).And the weight of offspring of CH group was lower than that of the group B and group C at P40(P<0.05).Compared with other groups,the control group had a significant decrease in the escape latency,which is steady and progressive.The distribution of the cortical and hippocampal nerve cells were disordered with unclear boundaries.Conclusion The established model rats reveal that the offspring of the SCH rats have abnormal weight and weakened learning and memory ability,while the conditions can be ameliorated by the L-T4 treatment before E13.

关 键 词：左旋甲状腺素 亚临床甲减 仔鼠 

分 类 号：R917[医药卫生—药物分析学]