离子色谱法测定岩藻聚糖中L-岩藻糖  

Determination of L-fucose in fucoidan by ion chromatography

作　　者：陈伟珠 方华 张怡评 晋文慧 陈晓莹 洪专 CHEN Weizhu;FANG Hua;ZHGNG Yiping;JIN Wenhui;CHEN Xiaoying;HONG Zhuan(Third Institute of Oceanography,Ministry of Natural Resource,Engineering Research Center of Marine Biological Resource Comprehensive Utilization,State Oceanic Administration,Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Xiamen 361005,China)

机构地区：[1]自然资源部第三海洋研究所,自然资源部海洋生物资源综合利用工程技术研究中心,福建省海洋生物资源开发利用协同创新中心,福建厦门361005

出　　处：《化学分析计量》2020年第5期19-21,共3页Chemical Analysis And Meterage

基　　金：国家重点研发计划项目(2016YFF0201104);海洋中试技术研发与检测公共服务平台建设(Bhsfs009);厦门海洋高技术产业基地创业创新共享服务平台项目(16PFW008SF15)。

摘　　要：建立测定岩藻聚糖中L-岩藻糖的离子色谱方法。对包括色谱柱、流动相等色谱条件进行了优化,选用DIONEX CarboPac PA10型(250 mm×4 mm)色谱柱,以H2O-250 mmol/L NaOH溶液(90∶10)为流动相,流量为1.00 mL/min,柱温为30℃,用脉冲安培检测器检测。在该条件下,L-岩藻糖的质量浓度在0.11~44.80μg/mL范围与色谱峰面积线性良好,相关系数为0.9924,检出限为0.05μg/mL,定量限为0.10μg/mL。样品加标平均回收率为91.79%,相对标准偏差为2.77%(n=6)。该法检测岩藻聚糖中L-岩藻糖,操作简单、快速,灵敏度高,测定结果准确。An ion chromatographic method was developed for the determination of L-fucose in fucoidan.After the optimization of chromatographic conditions such as column,mobile phase and so on were optimized,the samples were operated on DIONEX CarboPac PA10 Column(250 mm×4 mm)by using a mixture of H2O and 250 mmol/L sodium hydroxide solution(90∶10)as the mobile phase with the flow rate of 1.00 mL/min.The column temperature was 30℃and the detector was pulse ampere detector.A good linearity was observed in the concentration range of 0.11-44.80μg/mL with the correlation coefficient of 0.9924.The detection limit and the quantitative limit were 0.05μg/mL and 0.10μg/mL,respectively.The average recovery of L-fucose was 91.79%with the relative standard deviation of 2.77%(n=6).The results indicated that ion chromatographic method was a simple,fast,sensitive,accurate and effective method for the detection of L-fucose in fucoidan.

关 键 词：岩藻聚糖 L-岩藻糖 离子色谱法 测定 

分 类 号：O657.7[理学—分析化学]