Process Optimization and Content Determination of Total Flavonoids of Zhuang Medicine Clerodendrum japonicum(Thumb.)Sweet  

作　　者：Jiangcun WEI Zujie QIN Yunli TANG Nong TANG Liping QIN Yan MA Mingchan WEI Fengxian ZHAO 

机构地区：[1]Guangxi International Zhuang Medical Hospital, Nanning 530201, China [2]Guangxi Key Laboratory of Basic Research in Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530200, China [3]Guangxi University of Chinese Medicine, Nanning 530200, China

出　　处：《Medicinal Plant》2020年第4期44-47,53,共5页药用植物：英文版

基　　金：National Natural Science Foundation of China(81660659);Natural Science Foundation Project of Guangxi(2019GXNSF AA245090&2018GXNSFAA050141);Open Research Project of Guangxi Key Laboratory of Basic Research in Traditional Chinese Medicine(KJT1701205);Systematic Research Program of Chinese Medicine(Zhuang Medicine),a Key Discipline with Advantages and Characteristics in Guangxi:Research on the Production Process and Quality Control Method of Heyin Capsule;Program of Scientific Research Third-level Laboratory"Chinese(Zhuang)Medicine Chemical and Quality Analysis Laboratory"of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa 2009[21])。

摘　　要：[Objectives]To optimize the extraction process of total flavonoids of Zhuang medicine Clerodendrum japonicum(Thumb.)Sweet and establish a method for its extraction and content determination.[Methods]The total flavonoids of C.japonicum were extracted by reflux extraction method.Through a single factor experiment,the effects of extraction method,extraction solvent concentration,extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of C.japonicum.[Results]The optimal extraction process of the total flavonoids of C.japonicum was 75%ethanol,1∶20 solid-to-liquid ratio,and 50 min reflux extraction time.[Conclusions]This method can effectively determine the content of total flavonoids in C.japonicum and provide a certain scientific basis for the study of the quality standard of this Zhuang medicine.This method has high reproducibility.It is stable and feasible in extraction of total flavonoids from Zhuang medicine C.japonicum.

关 键 词：Clerodendrum japonicum(Thumb.)Sweet Total flavonoids Extraction process UV-visible spectrophotometry 

分 类 号：R28[医药卫生—中药学]