艾纳香内生真菌粉红粘帚霉抗菌次生代谢产物  

Antibacterial secondary metabolites of Clonostachys rosea,an endophytic fungus from Blumea balsamifera(L.)DC.

作　　者：舒雪纯 张影波 官玲亮[2] 陈振夏[2] 黄梅[2] 陈晓鹭 袁媛 元超 Xuechun Shu;Yingbo Zhang;Lingliang Guan;Zhenxia Chen;Mei Huang;Xiaolu Chen;Yuan Yuan;Chao Yuan(College of Forestry,Hainan University,Haikou 570228,Hainan,China;Tropical Crops Genetic Resources Institute,CATAS/Hainan Provincial Engineering Research Center for Tropical Medicinal Plant,Danzhou 571737,Hainan,China;School of Biosciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,China)

机构地区：[1]海南大学林学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南省热带药用植物工程研究中心,海南儋州571737 [3]广东药科大学生命科学与生物制药学院,广东广州510006

出　　处：《生物工程学报》2020年第8期1650-1658,共9页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.81560624);中国热带农业科学院基本科研业务费专项资金(No.1630032019045);海南省自然科学基金(No.219MS079)资助。

摘　　要：植物内生真菌一直是发现结构新颖、活性广泛的化合物的重要宝库。文中对一株艾纳香内生真菌粉红粘帚霉Clonostachysrosea进行化学研究,通过活性跟踪手段,结合硅胶柱色谱、凝胶色谱、以及半制备液相色谱技术从该菌株的发酵提取物中共分离获得6个单体化合物,经波谱学技术结合质谱鉴定其分别为verticillin A(1)、(S)-(+)-fusarinolic acid(2)、8-hydroxyfusaric acid(3)、cerebroside C(4)、3-Maleimide-5-oxime(5)以及bionectriol A(6)。所有化合物进行了体外抗大肠埃希菌、金黄色葡萄球菌、枯草芽孢杆菌以及铜绿假单胞菌4株细菌的活性评价,其中1、4和6对3株细菌表现出显著的抑菌活性,MIC值2-16μg/mL。研究结果为从黎药植物艾纳香的内生真菌中寻找新型抗生素提供重要参考。Endophytic fungus is an important treasure trove for discovery of structurally unusual and biologically diverse compounds.A phytochemical investigation on a fungus Clonostachys rosea inhabits inner tissue of Blumea balsamifera(L.)DC.was initiatedrecently in our lab.Six pure compounds were isolated through silica gel column chromatography,sephadex LH-20,and semi-preparative HPLC techniques,with bio-guided strategy.Their structures were characterized as verticillin A(1),(S)-(+)-fusarinolic acid(2),8-hydroxyfusaric acid(3),cerebroside C(4),3-Maleimide-5-oxime(5),and bionectriol A(6)by analyses of NMR and MS data.All compounds were tested in vitro antibacterial activities against four strains of bacteria,Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Pseudomonas aeruginosa,and results revealed that 1,4 and 6 display notableinhibition againstthree bacteria,with MIC values ranging from 2 to 16μg/mL.Our findings provide references for mining novel antibiotics from endophytes originated from Li Minority medicinal plant B.balsamifera(L.)DC.

关 键 词：艾纳香 内生真菌 次生代谢产物 抗菌活性 

分 类 号：Q935[生物学—微生物学]