蛹虫草菌生物合成虫草素的研究进展  

Advances in biosynthesis of cordycepin from Cordyceps militaris

作　　者：赵星月 李倩[2] 刘文静[1] 关海晴 李成[1] 王际辉[1,3] 王亮 Xingyue Zhao;Qian Li;Wenjing Liu;Haiqing Guan;Cheng Li;Jihui Wang;Liang Wang(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,Liaoning,China;School of Life Sciences and Biotechnology,Dalian University,Dalian 116622,Liaoning,China;School of Chemical Engineering and Energy Technology,Dongguan University of Technology,Dongguan 523808,Guangdong,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连大学生命科学与技术学院,辽宁大连116622 [3]东莞理工学院化学工程与能源技术学院,广东东莞523808

出　　处：《生物工程学报》2020年第7期1293-1304,共12页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(No.2018YFC1406800);大连市科技创新基金(No.2019J12SN59);辽宁省自然科学基金(No.2019-ZD-0575);辽宁省教育厅科研项目(No.J2020102)资助。

摘　　要：蛹虫草Cordyceps militaris是我国传统的药用真菌,虫草素是蛹虫草的主要活性成分,具有抗癌、抗肿瘤、抗病毒等多种生理功能。蛹虫草菌液体发酵是最有希望实现高效生产虫草素的途径,但现阶段生产强度低,亟需应用发酵工程及代谢工程手段提高虫草素产量。文中对液体发酵体系中培养基组分(碳/氮源、前体物质、金属离子等)和培养条件(pH、溶氧量、光照等)对虫草素产量的影响进行了总结,并对虫草素的分离纯化、生物合成基因簇及合成代谢途径进行了阐述,最后探讨了实现虫草素高效生产的关键环节。Cordycepin as the main active ingredient of Cordyceps militaris,a traditional medicinal fungus in China,has many physiological functions such as anti-cancer,anti-tumor and anti-virus activity.The most potential route for effective cordycepin production has been considered as liquid fermentation of C.militaris though with low productivity at present.Thus,it is urgent to apply both process engineering strategy and metabolic engineering strategy to enhance the productivity of cordycepin.In this review,the effects of medium components(i.e.the carbon/nitrogen source,precursor substances and metal ions)and operation factors(i.e.pH,dissolved oxygen and light)on cordycepin biosynthesis in liquid fermentation system are summarized.Besides,separation of cordycepin,the gene cluster involved and predicted biosynthesis pathways of cordycepin are also discussed,providing possible solutions of finally realizing efficient production of cordycepin.

关 键 词：虫草素 蛹虫草菌 液体发酵 生物合成途径 高效生产 

分 类 号：S567.3[农业科学—中草药栽培]