晚期氧化蛋白产物通过活性氧途径促进肝细胞凋亡  

Advanced oxidation protein products induce apoptosis of hepatocytes via the ROS-dependent pathway

作　　者：孙世波[1] 谢芳[2] 彭丽珊 冯伟标 梁可一 周杰[1] SUN Shi-bo;XIE Fang;PENG Li-shan;FENG Wei-biao;LIANG Ke-yi;ZHOU Jie(Department of Hepatobiliary Surgery,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China;Guangdong Provincial Key Laboratory of Gastroenterology,Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China Southern;Medical University,Guangzhou,Guangdong 510515,China)

机构地区：[1]南方医科大学南方医院肝胆外科,510515 [2]南方医科大学南方医院消化内科,510515 [3]南方医科大学,510515

出　　处：《现代消化及介入诊疗》2020年第8期1046-1050,1054,共6页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：国家自然科学基金青年项目(81800454);广东省自然科学基金(2018A030313219);广东省医学科研基金(A2018049)。

摘　　要：目的探讨晚期氧化蛋白产物(AOPP)对肝细胞凋亡的影响及其相关机制。方法体外制备AOPP处理体外培养的小鼠AML-12肝细胞,采用流式细胞仪检测细胞凋亡比例及细胞内活性氧(ROS)的变化,免疫组织化学法染色(Western blotting)检测凋亡蛋白3(caspase 3)以及裂解凋亡蛋白3(cleaved caspase 3)的表达水平。结果AOPP处理呈浓度依赖性激活caspase 3以及增加AML-12细胞凋亡数量;AOPP刺激可显著促进细胞内ROS生成,ROS清除剂预处理可缓解AOPP诱导细胞内ROS生成、caspase 3激活及肝细胞凋亡比例。结论AOPP通过ROS依赖性激活caspase 3途径,进而诱导肝细胞发生凋亡。Objective The study aims to explore the mechanism and impact of advanced oxidation protein products(AOPP)on hepatocyte apoptosis.Methods Rat liver cells(AμG-12)was disposed with AOPP in vitro.The effects of AOPP on hepatocyte apoptosis were evaluated by measuring apoptosis ratio,intracellular reactive oxygen species(ROS)through flow cytometry and expression of caspase 3,cleaved caspase 3 through Western blotting.Results AOPP treatment significantly up-regulated the expression of caspase 3 and ROS as well as AμG-12 apoptosis with dose-dependent.Pretreatment of ROS scavenger could reverse the AOPP-induced caspase 3,ROS and AμG-12 apoptosis increase.Conclusion AOPP induce hepatocyte apoptosis through ROSdependent activation of caspase 3.

关 键 词：晚期氧化蛋白产物 活性氧 凋亡蛋白3 肝细胞 

分 类 号：R602[医药卫生—外科学] R575[医药卫生—临床医学]