纵条纹炭角菌中环肽Xylastriamide A培养条件的优化  

Optimization of culture conditions of cyclopeptide Xylastriamide A in Xylaria striata

作　　者：苟欢 王莉[1] 范一婷 贺新生[1] 袁小红[1] Gou Huan;Wang Li;Fan Yiting;He Xinsheng;Yuan Xiaohong(School of Life Science and Engineering,Southwest University of Science And Technology,Mianyang,Sichuan 621010,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《食药用菌》2020年第5期309-313,共5页Edible and Medicinal Mushrooms

基　　金：国家自然科学基金项目(21272189);四川省大学生创新创业训练计划资助项目(201810619088);中国国家转基因重大专项(2019ZX08010-004)。

摘　　要：为优化纵条纹炭角菌中环肽Xylastriamide A的固体培养和液体培养条件,采用高效液相色谱法,测定分析固体培养培养料不同棉籽壳与麦麸比例配方,及液体培养不同碳源、氮源和培养时间的环肽Xylastriamide A含量差异。结果:固体培养,以培养料中的棉籽壳/麦麸比为70/30时,子实体的环肽Xylastriamide A含量较高,达578.20μg/g;液体培养,最佳碳源和最佳氮源分别为麦芽糖和酵母浸出粉,培养14天得到的菌丝球环肽Xylastriamide A含量分别达到9.77μg/g和19.95μg/g。The content of cyclopeptide Xylastriamide A in Xylaria striata was increased by optimizing the culture conditions.Determination of cyclopeptide Xylastriamide A in Xylaria striata by HPLC.The solid culture materials were optimized,and the content of cyclopeptide Xylastriamide A in different ratios of cottonseed hull and wheat bran of culture medium formulas were compared;and the liquid culture which was based on the basic medium was to compare the content of cyclopeptide Xylastriamide A in different carbon sources,nitrogen sources and culture time.The results showed that when the ratio of cottonseed hull to wheat bran was 70∶30 in culture medium formula,the content of cyclopeptide Xylastriamide A in fruiting body was as high as 578.20μg/g.In liquid culture,The best carbon source and nitrogen source were maltose and yeast powder,the Xylastriamide A content of mycelium after 14 days of culture was 9.77μg/g and 19.95μg/g,respectively.

关 键 词：纵条纹炭角菌 高效液相色谱法 固体培养 液体培养 

分 类 号：S646[农业科学—蔬菜学] R285[农业科学—园艺学]