思茅松花粉活力测定方法研究  

Selection of Measurements on Pollen Viability in Pinus kesiya var.langbianensis

作　　者：付强 陈伟 李江[1,3] 冯弦 陈绍安[1] 孟梦 付玉嫔 史富强 何俊 FU Qiang;CHEN Wei;LI Jiang;FENG Xian;CHEN Shao-an;MENG Meng;FU Yu-pin;SHI Fu-qiang;HE Jun(Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China;Forest Seed and Seedling Station of Pu’er Municipality,Pu’er Yunnan 665000,P.R.China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,Kunming Yunnan 650201,P.R.China;Forestry Research Institute of Pu’er Municipality,Pu’er Yunnan 665000,P.R.China)

机构地区：[1]云南省林业和草原科学院,云南昆明650201 [2]普洱市林木种苗工作站,云南普洱665000 [3]云南省森林植物培育与开发利用重点实验室,云南昆明650201 [4]普洱市林业科学研究所,云南普洱665000

出　　处：《西部林业科学》2020年第3期7-13,共7页Journal of West China Forestry Science

基　　金：云南省林业和草原科学院创新基金项目(MS2019-02)。

摘　　要：为探讨适于思茅松花粉活力快速检测的方法,以普文思茅松种子园的花粉为材料,采用离体萌发法、I2-KI染色法、TTC染色法、醋酸洋红染色法和FDA染色法对思茅松花粉活力进行测定。结果表明,采用离体萌发法测定思茅松花粉活力时,H3BO3溶液较CaCl2溶液更适于作为萌发培养基,以50mg/L浓度的H3BO3溶液为培养基,30℃条件下培养24h能测定出花粉活力;染色法以I2-KI染色效果最好,染色速度快,且染色率与萌发法检测的花粉活力的数据较为接近,无显著差异;TTC染色法和FDA染色法的效果次之,能对有活力的思茅松花粉染色,但测定的活力值与萌发法具有显著的差异,TTC染色法活力测定值仅为萌发法的32.8%,而FDA染色法活力测定值则较萌发法偏高34.2%;醋酸洋红染色法对思茅松花粉不能染色,不适宜思茅松花粉活力检测。In order to explore the method suitable for the rapid determination of pollen viability of Pinus kesiya var.langbianensis,in vitro germination method,I2-KI staining,TTC staining,aceto carmine staining and FDA staining were used to determine the pollen viability of P.kesiya var.langbianensis which from the seed orchard.The results showed that H3BO3 solution was more suitable than CaCl2 solution as the culture medium for determining the pollen germination in vitro,and the pollen viability could be measured by using H3BO3 solution at the concentration of 50 mg/L under the condition of 30℃for 24 h;I2-KI method was the best staining method with fast staining speed,and its staining rate was close to the data of pollen viability detected by germination method without significant difference;TTC staining and FDA staining were the next most effective,two methods could stain the active pollen of P.kesiya var.langbianensis,but the measured viability value was significantly different from that of the germination method,the TTC staining value was only 32.8%of that of the germination method,while the FDA staining value was 34.2%higher than that of the germination method;the method of aceto carmine staining was not suitable for the pollen activity detection of P.kesiya var.langbianensis.

关 键 词：思茅松 花粉活力 离体培养 染色法 

分 类 号：S791.259[农业科学—林木遗传育种]