文山兜兰种子非共生萌发成苗的研究  

Study on Seedlings of Non-symbiotic Germination of Paphiopedilum wenshanense

作　　者：华梅 孔继君[1,2] 向振勇 赵黎明[1] 蒋宏 HUA Mei;KONG Ji-jun;XIANG Zhen-yong;ZHAO Li-ming;JIANG Hong(Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China;Key Laboratory for Conservation of Rare,Endangered&Endemic Forest Plants,State Forestry Administration,Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,Kunming Yunnan 650201,P.R.China)

机构地区：[1]云南省林业和草原科学院,云南昆明650201 [2]国家林业和草原局云南珍稀濒特森林植物保护和繁育实验室/云南省森林植物培育与开发利用重点实验室,云南昆明650201

出　　处：《西部林业科学》2020年第3期56-59,73,共5页Journal of West China Forestry Science

基　　金：云南省林业科学院创新基金项目-极小种群文山兜兰的组织培养与快速繁殖技术研究(QN2018-02);云南省兰科植物资源调查(2019073017);国家林业和草原局野生动植物保护项目-文山兜兰野外种群拯救与繁育回归。

摘　　要：采用液体悬浮培养的方法对文山兜兰种子进行萌发培养,以花宝1号(1g/L)+C(0.5g/L)+椰子粉(10g/L)+琼脂(8g/L)+蔗糖(20g/L)+6-BA(1.0mg/L)+NAA(2.0mg/L)和花宝1号(1g/L)+C(0.5g/L)+椰子粉(10g/L)+琼脂(8g/L)+蔗糖(20g/L)+6-BA(2.0mg/L)+NAA(1.0mg/L)分别作为壮苗和生根培养基对文山兜兰幼苗进行培养。结果表明,采用液体悬浮培养的方式,文山兜兰种子萌发的时间为(59±2.67)d,平均萌发率为(66.33±6.22)%。采用该壮苗和生根培养基,文山兜兰幼苗生长健壮,并且根系发达、粗壮,成活率高。通过炼苗方法操作,在水苔基质中,移栽后的幼苗成活率高达90%以上。The seed culture of Paphiopedilum wenshanense was germinated by liquid suspension culture,with Huabao No.1(1 g/L)+C(0.5 g/L)+coconut powder(10 g/L)+8 g/L agar+20 g/L sucrose+6-BA 1.0 mg/L+NAA 2.0 mg/L and Huabao 1(1 g/L)+C(0.5 g/L)+coconut powder(10 g/L)+8 g/L agar+20 g/L sucrose+6-BA 2.0 mg/L+NAA 1.0 mg/L as a strong seedling and rooting medium for the cultivation of P.wenshanense seedlings.The results showed that the using of liquid suspension culture,the germination time of P.wenshanense was(59±2.67)d,and the average germination rate was(66.33±6.22)%.Using the strong seedling and rooting medium,the seedlings of P.wenshanense grow robustly,and the roots were developed,thick,and had a high survival rate.Through the operation of the seedling method,the survival rate of the seedlings after transplanting was as high as 90%or more in the moss substrate.

关 键 词：文山兜兰 非共生萌发 无菌苗 

分 类 号：S682.31[农业科学—观赏园艺]