标本处理方法对甲状旁腺激素检测的影响  

Effect of specimen treatment method on detection of parathyroid hormone

作　　者：王路长[1] 魏晓玲[1] 贺晨[1] 游婷 胡莹 WANG Luchang;WEI Xiaoling;HE Cheng;YOU Ting;HU Ying(Department of Clinical Laboratory,Chengdu Municipal Second People′s Hospital,Chengdu,Sichuan 610017,China)

机构地区：[1]四川省成都市第二人民医院检验科,四川成都610017

出　　处：《检验医学与临床》2020年第18期2661-2664,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨不同的血液标本及处理方法在日本东曹AIA2000全自动化学发光分析仪上测定甲状旁腺激素(PTH)水平的差异,以选择合适的上机标本类型及处理办法。方法用血清管、肝素抗凝管(肝素管)、乙二胺四乙酸二钾(EDTA-K 2)抗凝管(EDTA管)3种不同的真空采血管采集同一患者血液,每一患者每种试管各采3管,共10例患者纳入研究,均在2 h内测定iPTH水平;然后分3组,每组每例患者3种试管各一管,一组放于冷藏2~8℃,一组放冷冻-18^-22℃(分离血清或血浆),分别在第一次检测后8、24 h及72 h测定;另外一组,放于室温(18~25℃),分别在第一次测定后2、4、8、24 h进行检测;最后对所有数据进行统计分析。结果所有标本在2 h内的测定结果,血清管与EDTA管、肝素管与EDTA管比较差异均无统计学意义(P>0.05)。血清管的结果在室温、冷藏下均随着时间的推移逐渐降低,2、4、8、24 h的检测结果与0 h比较,差异均有统计学意义(P<0.05)。肝素管的结果在室温下4 h内结果差异无统计学意义(P>0.05),8 h后结果下降,8、24 h的检测结果与0 h比较,差异有统计学意义(P<0.05);肝素管在冷藏下72 h内结果稳定性较好,8、24、72 h的检测结果与0 h比较,差异无统计学意义(P>0.05)。EDTA管的结果在室温下24 h内结果差异无统计学意义(P>0.05),在冷藏下72 h内结果差异无统计学意义(P>0.05)。血清管、EDTA管和肝素管在冷冻保存下,8、24、72 h的检测结果均较0 h降低,差异均有统计学意义(P<0.05),但是在冷冻8 h后,随着时间增加降低效果不明显。结论在日本东曹AIA2000全自动化学发光分析仪上测定PTH最好使用EDTA抗凝标本,如果使用肝素标本,需放于2~8℃保存,可保存72 h,血清标本则必须在2 h内尽快进行测定分析。Objective To investigate the difference of different blood specimens and treatment methods for detecting the parathyroid hormone(PTH)level on the TOSOH AIA2000 automatic chemiluminescence analyzer in order to select the appropriate the specimen type and treatment method on the machine.Methods The three different kinds of vacuum blood collection tubes serum tubes,heparin anticoagulant tubes,and dipotassium ethylenediamine tetraacetate(EDTA-K 2)anticoagulant tubes,were used to collect the blood of the same patient.Each kind of tube collected each 3 tubes of blood in each patient.A total of 10 patients were included into the study and the iPTH level was measured within 2 h.Then the patients were divided into the three groups,each case in each group had 1 tube among 3 kinds of test tubes.One group was put for cold storage at 2-8℃and the another group was put for freezing at-18--22℃(separating serum or plasma),the detection was conducted at 8,24,72 h after the first detection;the another group was placed in the room temperature(18-25℃),the detection was conducted at 2,4,8,24 h after the first detection;finally the whole data conducted the statistical analysis.Results All specimens were detected within 2 h.There was no statistically significant difference between the serum tube and EDTA tube and between the heparin tube and EDTA tube(P>0.05).The results of serum tubes were decreased gradually with the time lapse at room temperature and under cold storage.The results at 2,4,8,24 h had statistically significant difference compared with those at 0 h(P<0.05).There was no statistically significant difference in the results of heparin tubes under the room temperature within 4 h(P>0.05),but the results were decreased after 8 h.The detection results of heparin tubes had statistical difference between at 8 h and 24 h with 0 h(P<0.05).The detection results of heparin tube under the room temperature within 4 h had no statistical difference,the results after 8 h were decreased,the detection results had statistical difference

关 键 词：甲状旁腺激素 标本类型 标本处理 抗凝剂 

分 类 号：R446[医药卫生—诊断学]