HPLC法测定乙肝宁颗粒中鸡矢藤次苷甲酯、车叶草苷酸、车叶草苷、丹参素、丹酚酸B、丹参酮ⅡA、毛蕊异黄酮葡萄糖苷和芒柄花素  

Determination of feretoside,asperulosidic acid,asperuloside,danshensu,salvianolic acid B,tanshinoneⅡA,calycosin 7-O-β-D-glucopyranoside,and formononetin in Yiganning Granules by HPLC

作　　者：李季梅 唐铖 高森[3] LI Ji-mei;TANG Cheng;GAO Sen(Department of Pharmacy,Tianjin Ninghe Hospital,Tianjin 301500,China;Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics(Theranostics),College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Department of Pharmacy,General Hospital,Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]天津市宁河区医院药剂科,天津301500 [2]天津医科大学药学院天津市临床药物关键技术重点实验室,天津300070 [3]天津医科大学总医院药剂科,天津300052

出　　处：《现代药物与临床》2020年第8期1527-1531,共5页Drugs & Clinic

基　　金：天津市自然科学基金资助项目(18JCYBJC95100)。

摘　　要：目的建立HPLC法同时测定乙肝宁颗粒中鸡矢藤次苷甲酯、车叶草苷酸、车叶草苷、丹参素、丹酚酸B、丹参酮ⅡA、毛蕊异黄酮葡萄糖苷和芒柄花素。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–0.1%甲酸溶液,梯度洗脱;检测波长分别为238 nm(0~21.0 min检测鸡矢藤次苷甲酯、车叶草苷酸和车叶草苷)、280 nm(21.0~40.0 min检测丹参素、丹酚酸B和丹参酮ⅡA)和254 nm(40.0~55.0 min检测毛蕊异黄酮葡萄糖苷和芒柄花素);体积流量:1.0 mL/min;柱温:30℃;进样量为10μL。结果鸡矢藤次苷甲酯、车叶草苷酸、车叶草苷、丹参素、丹酚酸B、丹参酮ⅡA、毛蕊异黄酮葡萄糖苷和芒柄花素分别在1.49~37.25、0.76~19.00、1.07~26.75、0.99~24.75、12.61~315.25、2.43~60.75、0.69~17.25、1.86~46.50μg/mL线性关系良好(r≥0.9991);各成分平均加样回收率分别为98.44%、98.12%、99.36%、97.44%、100.00%、99.19%、96.98%、98.93%,RSD值分别为0.71%、1.47%、0.98%、1.35%、0.85%、1.13%、1.02%、1.29%。结论该方法操作简便、重复性好,可用于乙肝宁颗粒中多指标性成分的质量控制。Objective To establish an HPLC method for simultaneous determination of feretoside,asperulosidic acid,asperuloside,danshensu,salvianolic acid B,tanshinoneⅡA,calycosin 7-O-β-D-glucopyranoside and formononetin in Yiganning Granules.Methods The chromatographic separation was carried out on Kromasil C18 column(250 mm×4.6 mm,5μm)with the mobile phase consisting of acetonitrile-0.1%formic acid solution with gradient elution at a flow rate of 1.0 mL/min.The column temperature was 30℃.The detection wavelength were set at 238 nm for feretoside,asperulosidic acid and asperuloside,280 nm for danshensu,salvianolic acid B and tanshinoneⅡA,and 254 nm for calycosin 7-O-β-D-glucopyranoside and formononetin.Results Feretoside,asperulosidic acid,asperuloside,danshensu,salvianolic acid B,tanshinoneⅡA,calycosin 7-O-β-D-glucopyranoside and formononetin showed good linear relationships within the ranges of 1.49—37.25,0.76—19.00,1.07—26.75,0.99—24.75,12.61—315.25,2.43—60.75,0.69—17.25,1.86—46.50μg/mL(r≥0.9991),whose average recoveries were 98.44%,98.12%,99.36%,97.44%,100.00%,99.19%,96.98%,and 98.93%with the RSD values of 0.71%,1.47%,0.98%,1.35%,0.85%,1.13%,1.02%,and 1.29%,respectively.Conclusion The method is easy to operate and repeatable,can be used for quality control of multiple indicator components in Yiganning Granules.

关 键 词：乙肝宁颗粒 鸡矢藤次苷甲酯 车叶草苷酸 车叶草苷 丹参素 丹酚酸B 丹参酮ⅡA 毛蕊异黄酮葡萄糖苷 芒柄花素 高效液相色谱 

分 类 号：R284.02[医药卫生—中药学]