Abnormal Karyotype of Pen Shell (Atrina pectinata) During Its Early Embryonic Development in Late Breeding Season  

作　　者：ZHOU Liqing WANG Xuemei YANG Aiguo WU Biao SUN Xiujun LIU Zhihong CHEN Siqing ZHAO Dan 

机构地区：[1]Key Laboratory for Sustainable Development of Marine Fisheries of Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Qingdao 266071,China [2]Laboratory for Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China [3]Rizhao Ocean and Fisheries Research Institute,Rizhao 276826,China [4]College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Journal of Ocean University of China》2020年第4期902-910,共9页中国海洋大学学报（英文版）

基　　金：part by the Central Public-Interest Scientific Institution Basal Research Fund,YSFRI,CAFS,China(No.20603022018004);the National Natural Science Foundation of China(No.31672637);the National Key R&D Program of China(No.2018YFD0900800);the Key Research and Development Plan of Shandong Province,China(No.2016GSF115012).

摘　　要：The pen shell,Atrina pectinata,distributes globally.It is one of the most important edible bivalves in east Asian countries.However,there are multiple difficulties in rearing pen shell larvae and juveniles because of their high mortality.To understand the mechanism underlining such high mortality at the early embryonic development stage,we obtained approximately 100 million larvae during later breeding season in June,2017,and checked a large amount of mitotic chromosomal plates of the early embryos and post-spawning gonads tissue slices of their parents.The results showed that most diploid A.pectinata embryos have 17 pairs of chromosomes(2n=34)as their parents do.The first pair of particularly large chromosomes are heterotypic in some diploid embryos while they are homomorphic in others.The primary sex-determination chromosome type is XX/XY.A lot of triploid,pentaploid and aneuploid embryos with different numbers of the largest homomorphic or heteromorphic chromosomes were found due to the degeneration of overmatured parent gonads which hold normal karyotype.These larvae will die even though most of them may develop into the trochophore stage with 34 chromosomes.Genetic deficiency of chromosomes will cause a high rate of mortality in early embryos in late breeding season.These findings should enrich the current knowledge of juvenile pen shell aquaculture.

关 键 词：Atrina pectinata early embryo sex chromosome nuclear ploidy later breeding season 

分 类 号：S917.4[农业科学—水产科学]